Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein

Elements that contribute to the high, stable yield of soybean peroxidase (SBP) in soybean seed coats can be exploited in the development of this tissue as a protein production platform. SBP contains an N-terminal and a C-terminal propeptide that are predicted to direct vacuolar targeting; this may b...

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Bibliographic Details
Published in:Plant cell reports 2010-09, Vol.29 (9), p.987-996
Main Authors: Schnell, Jaimie A, Han, Shuyou, Miki, Brian L, Johnson, Douglas A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis - metabolism
Biomedical and Life Sciences
Biotechnology
C-terminal propeptide
Cell Biology
Endoplasmic Reticulum - metabolism
Glycine max - enzymology
Growth conditions
Life Sciences
Molecular Sequence Data
N-terminal propeptide
Original Paper
Peptides
Peroxidases - genetics
Peroxidases - metabolism
Plant Biochemistry
Plant Leaves - enzymology
Plant Sciences
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Protein Precursors - genetics
Protein Precursors - metabolism
Protein Sorting Signals
signal peptide
Soybean peroxidase
Soybeans
testa
vacuoles
Vacuoles - metabolism
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Summary:Elements that contribute to the high, stable yield of soybean peroxidase (SBP) in soybean seed coats can be exploited in the development of this tissue as a protein production platform. SBP contains an N-terminal and a C-terminal propeptide that are predicted to direct vacuolar targeting; this may be one factor that contributes to its high yield and stability. We characterized the function of the SBP propeptides and investigated their ability to increase the yield of a foreign protein in a heterologous plant system. SBP propeptides are functional signal peptides capable of directing vacuolar transport in Arabidopsis. The use of these propeptides as well as an endoplasmic reticulum (ER)-retention signal to direct a foreign protein to the apoplast, ER, or vacuole can significantly increase yield and will therefore be useful for the development of the seed coat as a protein production platform. We also demonstrate that growth conditions may have a significant impact on the yield of a foreign protein and that this may be subcellular compartment-specific.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-010-0884-y