Comparative quantitation of proteome alterations induced by aging or immortalization in primary human fibroblasts and keratinocytes for clinical applications

We monitor proteome changes of primary human skin fibroblasts and keratinocytes during cell culture and compare them to respective immortalized cell lines using stable isotope labeling by amino acids to address their suitability as cell models for clinical disease proteomics.

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Bibliographic Details
Published in:Molecular bioSystems 2010-09, Vol.6 (9), p.1579-1582
Main Authors: Sprenger, Adrian, Küttner, Victoria, Biniossek, Martin L, Gretzmeier, Christine, Boerries, Melanie, Mack, Claudia, Has, Cristina, Bruckner-Tuderman, Leena, Dengjel, Jörn
Format: Article
Language:English
Subjects:
Cells, Cultured
Cellular Senescence - physiology
Fibroblasts - metabolism
Humans
Keratinocytes - metabolism
Proteome - metabolism
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Description
Summary:We monitor proteome changes of primary human skin fibroblasts and keratinocytes during cell culture and compare them to respective immortalized cell lines using stable isotope labeling by amino acids to address their suitability as cell models for clinical disease proteomics.
ISSN:1742-206X
1742-2051
DOI:10.1039/c003962d