Role of cGMP-PKG signaling in the protection of neonatal rat cardiac myocytes subjected to simulated ischemia/reoxygenation

Nitric oxide (NO) and B-type natriuretic peptide (BNP) are protective against ischemia–reperfusion injury as they increase intracellular cGMP level via activation of soluble (sGC) or particulate guanylate cyclases (pGC), respectively. The aim of the present study was to examine if the cGMP-elevating...

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Bibliographic Details
Published in:Basic research in cardiology 2010-09, Vol.105 (5), p.643-650
Main Authors: Gorbe, Aniko, Giricz, Zoltan, Szunyog, Andrea, Csont, Tamas, Burley, Dwaine S., Baxter, Gary F., Ferdinandy, Peter
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Calcium-Binding Proteins - metabolism
Cardiology
Cell Survival - drug effects
Cell Survival - physiology
Cells, Cultured
Cyclic GMP - analogs & derivatives
Cyclic GMP - pharmacology
Cyclic GMP-Dependent Protein Kinases - metabolism
Enzyme Activation - drug effects
Enzyme Activation - physiology
Medicine
Medicine & Public Health
Myocardial Reperfusion Injury - metabolism
Myocardial Reperfusion Injury - pathology
Myocytes, Cardiac - cytology
Myocytes, Cardiac - metabolism
Natriuretic Peptide, Brain - metabolism
Nitric Oxide - metabolism
Nitric Oxide Donors - pharmacology
Original Contribution
Phosphorylation - drug effects
Phosphorylation - physiology
Rats
Rats, Wistar
Signal Transduction - drug effects
Signal Transduction - physiology
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Summary:Nitric oxide (NO) and B-type natriuretic peptide (BNP) are protective against ischemia–reperfusion injury as they increase intracellular cGMP level via activation of soluble (sGC) or particulate guanylate cyclases (pGC), respectively. The aim of the present study was to examine if the cGMP-elevating mediators, NO and BNP, share a common downstream signaling pathway via cGMP-dependent protein kinase (PKG) in cardiac cytoprotection. Neonatal rat cardiac myocytes in vitro were subjected to 2.5 h simulated ischemia (SI) followed by 2 h reoxygenation. Cell viability was tested by trypan blue exclusion assay. PKG activity of cardiac myocytes was assessed by phospholamban (PLB) phosphorylation determined by western blot. Cell death was 34 ± 2% after SI/reoxygenation injury in the control group. cGMP-inducing agents significantly decreased irreversible cell injury: the cGMP analog 8-bromo-cGMP (8-Br-cGMP, 10 nM) decreased it to 13 ± 1% ( p  
ISSN:0300-8428
1435-1803
DOI:10.1007/s00395-010-0097-0