Age dependent incorporation of 14C-DHA into rat brain and body tissues after dosing various 14C-DHA-esters

• Published in: Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2010-08, Vol.83 (2), p.89-96
• Main Authors: Graf, B.A., Duchateau, G.S.M.J.E., Patterson, A.B., Mitchell, E.S., van Bruggen, P., Koek, J.H., Melville, S., Verkade, H.J.
• Format: Article
• Language: English
• Subjects: Aging - metabolism; Animals; Bioavailability; Biological and medical sciences; Biological Transport - drug effects; Biological Transport - physiology; Body distribution; Brain; Brain - metabolism; Brain Chemistry - drug effects; Brain Chemistry - physiology; Carbon Isotopes - metabolism; Carbon Isotopes - pharmacology; DHA; Docosahexaenoic Acids - metabolism; Docosahexaenoic Acids - pharmacology; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Humans; Male; Omega-3 fatty acids; Rats; Rats, Wistar; Time Factors; Vertebrates: endocrinology
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/j.plefa.2010.05.004

The omega-3 fatty acid docosahexaenoic acid (DHA) accounts for 10% of fatty acids in human brain and is critical for neuronal function and brain development. Mechanisms of transport, accumulation and conservation of DHA in the brain are unclear. The objective of the study was to quantify the age dependent DHA incorporation into the brain of 2-, 4- or 10-week-old rats after a bolus dose of different DHA-esters. Rats were gavaged with 14C-DHA-TAG, 14C-DHA-PL or 14C-DHA-TAG+PL at 2 mg DHA/kg BW. After 24 h the distribution of radioactivity in body and brain regions was determined using quantitative whole body autoradiography (QWBA). Radiolabeled compounds were extracted from the brains to determine the identity of the radiolabeled compounds. Accumulation of orally ingested 14C-DHA in rat brain was less than 1% of the dose and decreased with age. Ester specific differences were seen only in 10-week-old rats, where oral 14C-DHA-PL delivered a 2-fold higher accretion of radioactivity in the brain. Less than 1% of a dietary achievable DHA dose reached the rat brain within 24 h. Optimal efficacy of DHA-PL may occur in older age groups.