Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

RNA−coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA−protein interactions a...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2010-08, Vol.132 (32), p.11036-11038
Main Authors: Liu, Ningning, Peng, Bo, Lin, Yuan, Su, Zhaohui, Niu, Zhongwei, Wang, Qian, Zhang, Wenke, Li, Hongbin, Shen, Jiacong
Format: Article
Language:English
Subjects:
Hydrogen-Ion Concentration
Microscopy, Atomic Force
Models, Molecular
Molecular Conformation
RNA, Viral - chemistry
RNA, Viral - metabolism
Spectrum Analysis
Tobacco Mosaic Virus - chemistry
Tobacco Mosaic Virus - metabolism
Viral Proteins - chemistry
Viral Proteins - metabolism
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Summary:RNA−coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA−protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid−protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja1052544