Effect of retinoic acid on renal development in newborn mice treated with an angiogenesis inhibitor

Background:  A mouse model of impaired renal development was developed and the effect of retinoic acid (RA) was investigated in this animal model. Methods:  An angiogenesis inhibitor (SU1498) was injected s.c. into day 3 C57BL/6 newborn mice to create a model of arrested renal development. RA (2 mg/...

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Published in:Pediatrics international 2010-06, Vol.52 (3), p.386-392
Main Authors: Lee, Soo Jeong, Cho, Su Jin, Ju, Sun Young, Ryu, Kyung-Ha, Sung, Soon Hee, Park, Eun Ae
Format: Article
Language:English
Subjects:
Analysis of Variance
angiogenesis inhibitor
Angiogenesis Inhibitors - pharmacology
Animals
Animals, Newborn
Biopsy, Needle
Body Weight
Cinnamates - pharmacology
Developmental biology
Female
Immunohistochemistry
Inhibitor drugs
Kidney - drug effects
Kidney - growth & development
Kidney Cortex - drug effects
Kidney Cortex - growth & development
Kidney Glomerulus - drug effects
Kidney Glomerulus - growth & development
Kidneys
Mice
Mice, Inbred C57BL
Models, Animal
Organ Size - drug effects
Pediatrics
Random Allocation
retinoic acid
Rodents
Statistics, Nonparametric
Tretinoin - pharmacology
vascular endothelial growth factor
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Summary:Background:  A mouse model of impaired renal development was developed and the effect of retinoic acid (RA) was investigated in this animal model. Methods:  An angiogenesis inhibitor (SU1498) was injected s.c. into day 3 C57BL/6 newborn mice to create a model of arrested renal development. RA (2 mg/kg) was injected i.p. for 10 days. Morphometry and immunohistochemistry were done. Results:  Mice injected with SU1498 demonstrated deranged renal development in tubular structure and glomerular tuft area. Cortical thickness and area of glomerular tuft were significantly decreased after vascular endothelial growth factor (VEGF) inhibitor, and were significantly restored by RA. The length of capillary loops/glomerulus, the number of podocytes/glomerulus, and density of peritubular capillaries on CD31 immunostaining were significantly decreased by VEGF blocking and recovered by RA. Conclusions:  VEGF plays a major role in renal development, and RA reverses the inhibited development caused by an angiogenesis inhibitor.
ISSN:1328-8067
1442-200X
DOI:10.1111/j.1442-200X.2009.02959.x