Positive Effects of ATP on G-Quadruplex-Hemin DNAzyme-Mediated Reactions

• Published in: Analytical chemistry (Washington) 2010-07, Vol.82 (14), p.6148-6153
• Main Authors: Kong, De-Ming, Xu, Jing, Shen, Han-Xi
• Format: Article
• Language: English
• Subjects: Adenosine triphosphatase; Adenosine Triphosphate - chemistry; Analytical chemistry; Benzothiazoles - chemistry; Catalysis; Deoxyribonucleic acid; DNA; DNA, Catalytic - chemistry; DNA, Catalytic - metabolism; Enzymes; G-Quadruplexes; Hemin - chemistry; Hydrogen Peroxide - chemistry; Oxidation; Silver - analysis; Spectrophotometry, Ultraviolet; Sulfonic Acids - chemistry
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac100940v

Some G-quadruplex-hemin complexes can be used as peroxidase-mimicking DNAzymes, catalyzing H2O2-mediated reactions such as the oxidation of 2,2′-azinobis (3-ethylbenzothiozoline)-6-sulfonic acid (ABTS) by H2O2. However, some challenges, for example, the relatively low catalytic activity and the disproportionation of the reaction product ABTS·+, may seriously restrict further development and applications of these complexes. Here, we demonstrated the positive effect of adenosine triphosphate (ATP) on G-quadruplex-hemin DNAzyme-mediated catalytic reactions. The presence of ATP not only improved the catalytic activity of G-quadruplex-hemin DNAzymes, but also inhibited the disproportionation of ABTS·+. These observations may improve the performance of existing G-quadruplex-hemin DNAzyme-based chemical sensors, for example, the Ag+-detection method that uses G-quadruplex-hemin DNAzymes, and widen the application range of G-quadruplex-hemin DNAzymes. We also demonstrated that the phosphate groups, nucleobase, and sugar of ATP determine the reaction-promoting ability of ATP. These observations may be helpful in the design of highly efficient enhancers for G-quadruplex-hemin DNAzymes.