Orientation of the axial ligands and magnetic properties of the hemes in the triheme ferricytochrome PpcA from G. sulfurreducens determined by paramagnetic NMR

The geometry of the axial ligands of the hemes in the triheme cytochrome PpcA from Geobacter sulfurreducens was determined in solution for the ferric form using the unambiguous assignment of the NMR signals of the α-substituents of the hemes. The paramagnetic 13C shifts of the hemes can be used to d...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2010-08, Vol.584 (15), p.3442-3445
Main Authors: Morgado, Leonor, Saraiva, Ivo H., Louro, Ricardo O., Salgueiro, Carlos A.
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Cytochromes - chemistry
Dac 7
Desulfuromonas acetoxidans triheme cytochrome
Electron transfer
Geobacter
Geobacter - metabolism
Geobacter sulfurreducens triheme cytochrome encoded by gene GSU0612
Heme - chemistry
Heme electronic structure
heteronuclear single quantum coherence
HSQC
Hydrogen-Ion Concentration
Ligands
Magnetic Resonance Spectroscopy
Magnetics
Multiheme cytochrome c
NMR
NOESY
nuclear overhauser spectroscopy
Oxidation-Reduction
Paramagnetic shift
PpcA
TOCSY
total correlation spectroscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The geometry of the axial ligands of the hemes in the triheme cytochrome PpcA from Geobacter sulfurreducens was determined in solution for the ferric form using the unambiguous assignment of the NMR signals of the α-substituents of the hemes. The paramagnetic 13C shifts of the hemes can be used to define the heme electronic structure, the geometry of the axial ligands, and the magnetic susceptibility tensor. The latter establishes the magnitude and geometrical dependence of the pseudocontact shifts, which are crucial to warrant reliable structural constraints for a detailed structural characterization of this paramagnetic protein in solution.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2010.06.043