Separation of erythrocytes infected with murine malaria parasites in metrizamide gradients

Density gradients with metrizamide, a tri-iodinated benzamido derivative of glucose, have been used to separate erythrocytes infected with 3 species of murine plasmodia. Good separations were obtained of uninfected erythrocytes from erythrocytes containing parasites in different developmental stages...

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Bibliographic Details
Published in:Parasitology 1979-10, Vol.79 (2), p.267-275
Main Authors: Eugui, Elsie M., Allison, A. C.
Format: Article
Language:English
Subjects:
Animals
Carbon Dioxide - biosynthesis
Cell Separation - methods
Centrifugation, Density Gradient - methods
Erythrocytes - cytology
Erythrocytes - metabolism
Erythrocytes - parasitology
Female
Glucose - metabolism
Metrizamide
Mice
Mice, Inbred BALB C
Plasmodium
Species Specificity
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Summary:Density gradients with metrizamide, a tri-iodinated benzamido derivative of glucose, have been used to separate erythrocytes infected with 3 species of murine plasmodia. Good separations were obtained of uninfected erythrocytes from erythrocytes containing parasites in different developmental stages. With metrizamide solutions, the densities required for isopycnic separation can be obtained without hypertonicity or high viscosity, and the viability and those aspects of the metabolism of parasites and erythrocytes that we measured are not detectably modified by exposure to these solutions. This type of separation has many possible applications to biochemical and immunological investigations.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000053348