Evidence for DNA Synthesis in Pneumocystis carinii Trophozoites Treated with the β-1,3-Glucan Synthesis Inhibitor Pneumocandin L-693,989

Pneumocandins inhibit β-1,3-glucan synthesis preventing the development of Pneumocystis cysts that are absent from the lungs of treated rats. To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4′,6-diamidino-2-phenylindole (DAPI)- and DB...

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Bibliographic Details
Published in:The Journal of eukaryotic microbiology 2010-09, Vol.57 (5), p.447-448
Main Authors: WYDER, MICHAEL A, JOHNSTON, LAURA Q, KANESHIRO, EDNA S
Format: Article
Language:English
Subjects:
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiparasitic agents
beta-Glucans - antagonists & inhibitors
beta-Glucans - metabolism
Biological and medical sciences
Cell Nucleus - drug effects
Cell Nucleus - genetics
Cell Nucleus - metabolism
Cytochemistry
DAPI
DB181
DNA
DNA Replication - drug effects
Echinocandins - pharmacology
Female
Humans
Lung - microbiology
Medical sciences
Nucleic Acid Synthesis Inhibitors - pharmacology
nucleus
Pharmacology. Drug treatments
Pneumocystis carinii - drug effects
Pneumocystis carinii - genetics
Pneumocystis carinii - growth & development
Pneumocystis carinii - metabolism
Pneumocystis Infections - microbiology
Rats
Rats, Inbred Lew
Trophozoites - drug effects
Trophozoites - growth & development
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Summary:Pneumocandins inhibit β-1,3-glucan synthesis preventing the development of Pneumocystis cysts that are absent from the lungs of treated rats. To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4′,6-diamidino-2-phenylindole (DAPI)- and DB181-stained Pneumocystis carinii isolated from pneumocandin L-693-989-treated rats. Fluorescence intensities of trophozoite nuclei from drug-treated rats were greater than those of untreated controls, suggesting that DNA replication was not inhibited but that cytokinesis and perhaps karyokinesis were blocked.
ISSN:1066-5234
1550-7408
DOI:10.1111/j.1550-7408.2010.00502.x