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Evidence for DNA Synthesis in Pneumocystis carinii Trophozoites Treated with the β-1,3-Glucan Synthesis Inhibitor Pneumocandin L-693,989
Pneumocandins inhibit β-1,3-glucan synthesis preventing the development of Pneumocystis cysts that are absent from the lungs of treated rats. To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4′,6-diamidino-2-phenylindole (DAPI)- and DB...
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| Published in: | The Journal of eukaryotic microbiology 2010-09, Vol.57 (5), p.447-448 |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c4592-399b30b733a5812a8471db48dda75d67f59ff64c990fae6ad6f110458f47a6b33 |
| container_end_page | 448 |
| container_issue | 5 |
| container_start_page | 447 |
| container_title | The Journal of eukaryotic microbiology |
| container_volume | 57 |
| creator | WYDER, MICHAEL A JOHNSTON, LAURA Q KANESHIRO, EDNA S |
| description | Pneumocandins inhibit β-1,3-glucan synthesis preventing the development of Pneumocystis cysts that are absent from the lungs of treated rats. To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4′,6-diamidino-2-phenylindole (DAPI)- and DB181-stained Pneumocystis carinii isolated from pneumocandin L-693-989-treated rats. Fluorescence intensities of trophozoite nuclei from drug-treated rats were greater than those of untreated controls, suggesting that DNA replication was not inhibited but that cytokinesis and perhaps karyokinesis were blocked. |
| doi_str_mv | 10.1111/j.1550-7408.2010.00502.x |
| format | article |
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To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4′,6-diamidino-2-phenylindole (DAPI)- and DB181-stained Pneumocystis carinii isolated from pneumocandin L-693-989-treated rats. Fluorescence intensities of trophozoite nuclei from drug-treated rats were greater than those of untreated controls, suggesting that DNA replication was not inhibited but that cytokinesis and perhaps karyokinesis were blocked.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/j.1550-7408.2010.00502.x</identifier><identifier>PMID: 20738464</identifier><identifier>CODEN: JEMIED</identifier><language>eng</language><publisher>Malden, USA: Malden, USA : Blackwell Publishing Inc</publisher><subject>Animals ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antiparasitic agents ; beta-Glucans - antagonists & inhibitors ; beta-Glucans - metabolism ; Biological and medical sciences ; Cell Nucleus - drug effects ; Cell Nucleus - genetics ; Cell Nucleus - metabolism ; Cytochemistry ; DAPI ; DB181 ; DNA ; DNA Replication - drug effects ; Echinocandins - pharmacology ; Female ; Humans ; Lung - microbiology ; Medical sciences ; Nucleic Acid Synthesis Inhibitors - pharmacology ; nucleus ; Pharmacology. Drug treatments ; Pneumocystis carinii - drug effects ; Pneumocystis carinii - genetics ; Pneumocystis carinii - growth & development ; Pneumocystis carinii - metabolism ; Pneumocystis Infections - microbiology ; Rats ; Rats, Inbred Lew ; Trophozoites - drug effects ; Trophozoites - growth & development</subject><ispartof>The Journal of eukaryotic microbiology, 2010-09, Vol.57 (5), p.447-448</ispartof><rights>2010 The Author(s). Journal compilation © 2010 by the International Society of Protistologists</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4592-399b30b733a5812a8471db48dda75d67f59ff64c990fae6ad6f110458f47a6b33</citedby><cites>FETCH-LOGICAL-c4592-399b30b733a5812a8471db48dda75d67f59ff64c990fae6ad6f110458f47a6b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23227689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20738464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WYDER, MICHAEL A</creatorcontrib><creatorcontrib>JOHNSTON, LAURA Q</creatorcontrib><creatorcontrib>KANESHIRO, EDNA S</creatorcontrib><title>Evidence for DNA Synthesis in Pneumocystis carinii Trophozoites Treated with the β-1,3-Glucan Synthesis Inhibitor Pneumocandin L-693,989</title><title>The Journal of eukaryotic microbiology</title><addtitle>J Eukaryot Microbiol</addtitle><description>Pneumocandins inhibit β-1,3-glucan synthesis preventing the development of Pneumocystis cysts that are absent from the lungs of treated rats. To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4′,6-diamidino-2-phenylindole (DAPI)- and DB181-stained Pneumocystis carinii isolated from pneumocandin L-693-989-treated rats. Fluorescence intensities of trophozoite nuclei from drug-treated rats were greater than those of untreated controls, suggesting that DNA replication was not inhibited but that cytokinesis and perhaps karyokinesis were blocked.</description><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiparasitic agents</subject><subject>beta-Glucans - antagonists & inhibitors</subject><subject>beta-Glucans - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytochemistry</subject><subject>DAPI</subject><subject>DB181</subject><subject>DNA</subject><subject>DNA Replication - drug effects</subject><subject>Echinocandins - pharmacology</subject><subject>Female</subject><subject>Humans</subject><subject>Lung - microbiology</subject><subject>Medical sciences</subject><subject>Nucleic Acid Synthesis Inhibitors - pharmacology</subject><subject>nucleus</subject><subject>Pharmacology. Drug treatments</subject><subject>Pneumocystis carinii - drug effects</subject><subject>Pneumocystis carinii - genetics</subject><subject>Pneumocystis carinii - growth & development</subject><subject>Pneumocystis carinii - metabolism</subject><subject>Pneumocystis Infections - microbiology</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Trophozoites - drug effects</subject><subject>Trophozoites - growth & development</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkc1uEzEQx1cIREvhFWAviEs3-PtD4lKFNDSKClIbIXGxvLs2cdh4U3uXJrwBr8OD8Ew4bAgc8cUz9u__n9FMluUQjGA6r1cjSCkoOAFihEB6BYACNNo-yE6PHw9TDBgrKMLkJHsS4woAyBCEj7MTBDgWhJHT7Pvkq6uNr0xu25C_vb7Ib3a-W5roYu58_sGbft1Wu9ilvNLBeefy29Bulu231nUmpsToztT5veuWeRLmP38U8BwX06avtP_H7covXem6VOVgqn2dKswLJvG5FPJp9sjqJppnh_ssW1xObsfvivn76dX4Yl5UhEpUYClLDEqOsaYCIi0Ih3VJRF1rTmvGLZXWMlJJCaw2TNfMQggIFZZwzUqMz7JXg-8mtHe9iZ1au1iZptHetH1UnAgpscQokWIgq9DGGIxVm-DWOuwUBGq_B7VS-3Gr_bjVfg_q9x7UNkmfH4r05drUR-GfwSfg5QHQsdKNDdpXLv7lMEKcCZm4NwN37xqz--8G1GyySEGSF4Pcxc5sj3IdvijGMafq4_VUzT4RiMHlWM0S_2LgrW6V_hxSS4ubZIwBFAJhCvAvJmK7aw</recordid><startdate>201009</startdate><enddate>201009</enddate><creator>WYDER, MICHAEL A</creator><creator>JOHNSTON, LAURA Q</creator><creator>KANESHIRO, EDNA S</creator><general>Malden, USA : Blackwell Publishing Inc</general><general>Blackwell Publishing Inc</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201009</creationdate><title>Evidence for DNA Synthesis in Pneumocystis carinii Trophozoites Treated with the β-1,3-Glucan Synthesis Inhibitor Pneumocandin L-693,989</title><author>WYDER, MICHAEL A ; JOHNSTON, LAURA Q ; KANESHIRO, EDNA S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4592-399b30b733a5812a8471db48dda75d67f59ff64c990fae6ad6f110458f47a6b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antiparasitic agents</topic><topic>beta-Glucans - antagonists & inhibitors</topic><topic>beta-Glucans - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytochemistry</topic><topic>DAPI</topic><topic>DB181</topic><topic>DNA</topic><topic>DNA Replication - drug effects</topic><topic>Echinocandins - pharmacology</topic><topic>Female</topic><topic>Humans</topic><topic>Lung - microbiology</topic><topic>Medical sciences</topic><topic>Nucleic Acid Synthesis Inhibitors - pharmacology</topic><topic>nucleus</topic><topic>Pharmacology. Drug treatments</topic><topic>Pneumocystis carinii - drug effects</topic><topic>Pneumocystis carinii - genetics</topic><topic>Pneumocystis carinii - growth & development</topic><topic>Pneumocystis carinii - metabolism</topic><topic>Pneumocystis Infections - microbiology</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Trophozoites - drug effects</topic><topic>Trophozoites - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WYDER, MICHAEL A</creatorcontrib><creatorcontrib>JOHNSTON, LAURA Q</creatorcontrib><creatorcontrib>KANESHIRO, EDNA S</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WYDER, MICHAEL A</au><au>JOHNSTON, LAURA Q</au><au>KANESHIRO, EDNA S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for DNA Synthesis in Pneumocystis carinii Trophozoites Treated with the β-1,3-Glucan Synthesis Inhibitor Pneumocandin L-693,989</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>2010-09</date><risdate>2010</risdate><volume>57</volume><issue>5</issue><spage>447</spage><epage>448</epage><pages>447-448</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><coden>JEMIED</coden><abstract>Pneumocandins inhibit β-1,3-glucan synthesis preventing the development of Pneumocystis cysts that are absent from the lungs of treated rats. To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4′,6-diamidino-2-phenylindole (DAPI)- and DB181-stained Pneumocystis carinii isolated from pneumocandin L-693-989-treated rats. Fluorescence intensities of trophozoite nuclei from drug-treated rats were greater than those of untreated controls, suggesting that DNA replication was not inhibited but that cytokinesis and perhaps karyokinesis were blocked.</abstract><cop>Malden, USA</cop><pub>Malden, USA : Blackwell Publishing Inc</pub><pmid>20738464</pmid><doi>10.1111/j.1550-7408.2010.00502.x</doi><tpages>2</tpages></addata></record> |
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| subjects | Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antiparasitic agents beta-Glucans - antagonists & inhibitors beta-Glucans - metabolism Biological and medical sciences Cell Nucleus - drug effects Cell Nucleus - genetics Cell Nucleus - metabolism Cytochemistry DAPI DB181 DNA DNA Replication - drug effects Echinocandins - pharmacology Female Humans Lung - microbiology Medical sciences Nucleic Acid Synthesis Inhibitors - pharmacology nucleus Pharmacology. Drug treatments Pneumocystis carinii - drug effects Pneumocystis carinii - genetics Pneumocystis carinii - growth & development Pneumocystis carinii - metabolism Pneumocystis Infections - microbiology Rats Rats, Inbred Lew Trophozoites - drug effects Trophozoites - growth & development |
| title | Evidence for DNA Synthesis in Pneumocystis carinii Trophozoites Treated with the β-1,3-Glucan Synthesis Inhibitor Pneumocandin L-693,989 |
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