Rapidly spreading CTX-M-type β-lactamase-producing Proteus mirabilis in Japan

• Published in: International journal of antimicrobial agents 2010-10, Vol.36 (4), p.340-342
• Main Authors: Kanayama, Akiko, Iyoda, Takako, Matsuzaki, Kaoru, Saika, Takeshi, Ikeda, Fumiaki, Ishii, Yoshikazu, Yamaguchi, Keizo, Kobayashi, Intetsu
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial Typing Techniques; beta-Lactamases - biosynthesis; beta-Lactamases - genetics; beta-Lactams - pharmacology; Biological and medical sciences; Ciprofloxacin - pharmacology; CTX-M-type β-lactamase; DNA, Bacterial - genetics; Drug Resistance, Bacterial; Electrophoresis, Gel, Pulsed-Field; Extended-spectrum β-lactamase; Humans; Infectious Disease; Japan - epidemiology; Medical sciences; Microbial Sensitivity Tests; Molecular Epidemiology; Pharmacology. Drug treatments; Polymerase Chain Reaction; Prevalence; Proteus Infections - drug therapy; Proteus Infections - epidemiology; Proteus Infections - microbiology; Proteus Infections - transmission; Proteus mirabilis; Proteus mirabilis - drug effects; Proteus mirabilis - enzymology; Proteus mirabilis - genetics; Quinolones - pharmacology; Sequence Analysis, DNA; Time Factors
• ISSN: 0924-8579; 1872-7913
• DOI: 10.1016/j.ijantimicag.2010.06.002

Abstract In recent years, increased isolation of extended-spectrum β-lactamase (ESBL)-producing Proteus mirabilis has been reported in Japan. We undertook an investigation to determine the prevalence of ESBL-producing P. mirabilis isolated in Japan and to characterise the genotype. Seventy-four P. mirabilis isolates recovered from specimens at 54 hospitals in Japan between March and October 2006 were included in the study. Of the 74 P. mirabilis isolates examined, 28 (37.8%) were ESBL-producers. The blaCTX-M-2 gene was found in 27 isolates, whilst 1 isolate possessed blaCTX-M-3 . Amongst the 28 ESBL-producers, 25 (89.3%) were non-susceptible to ciprofloxacin, whilst 11 (23.9%) of 46 ESBL-non-producing isolates were non-susceptible to ciprofloxacin. Pulsed-field gel electrophoresis (PFGE) analysis of the 28 ESBL-producing isolates from 19 hospitals revealed 17 clusters. The same PFGE type was observed in two or more hospitals especially in the greater Tokyo area, suggesting possible clonal spread and the need for monitoring to determine whether emergence of a dominant clone occurs. Our results show that in Japan there is a high prevalence of CTX-M-type β-lactamase-producing P. mirabilis . Moreover, these isolates are characterised by reduced susceptibility to fluoroquinolones.