Morphological characterization of periodontium-derived human stem cells

The aim of this study has been to characterize adult human somatic periodontium-derived stem cells (PDSCS) isolated from human periodontium and to follow their differentiation after cell culture. PDSCS were isolated from human periodontal tissue and cultured as spheres in serum-free medium. After 10...

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Bibliographic Details
Published in:Annals of anatomy 2010-08, Vol.192 (4), p.215-219
Main Authors: Arnold, Wolfgang H., Becher, Sebastian, Dannan, A., Widera, Darius, Dittmar, T., Jacob, Monica, Mannherz, Hans Georg, Dittmar, Thomas, Kaltschmidt, B., Kaltschmidt, C., Grimm, Wolf-Dieter
Format: Article
Language:English
Subjects:
Adult
Adult Stem Cells - cytology
Adult Stem Cells - ultrastructure
Cell Culture Techniques
Cell Differentiation
Cell Shape
Cell Surface Extensions - ultrastructure
Cells, Cultured
Culture Media, Serum-Free
Differentiation
Extracellular matrix
Extracellular Matrix - chemistry
Extracellular Matrix - ultrastructure
Humans
Morphology
Osteopontin
Osteopontin - analysis
Periodont
Periodontal Ligament - cytology
Pseudopodia - ultrastructure
Stem cells
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Summary:The aim of this study has been to characterize adult human somatic periodontium-derived stem cells (PDSCS) isolated from human periodontium and to follow their differentiation after cell culture. PDSCS were isolated from human periodontal tissue and cultured as spheres in serum-free medium. After 10 days the primary spheres were dissociated and the secondary spheres sub-cultured for another 1–2 weeks. Cells from different time points were analyzed, and immunohistochemical and electron microscopic investigations carried out. Histological analysis showed differentiation of spheres deriving from the PDSCS with central production of extracellular matrix beginning 3 days after sub-culturing. Isolated PDSCS developed pseudopodia which contained actin. Tubulin was found in the central portion of the cells. Pseudopodia between different cells anastomosed, indicating intercellular transport. Immunostaining for osteopontin demonstrated a positive reaction in primary spheres and within extracellular matrix vesicles after sub-culturing. In cell culture under serum-free conditions human PDSCS form spheres which are capable of producing extracellular matrix. Further investigations have do be carried out to investigate the capability of these cells to differentiate into osteogenic progenitor cells.
ISSN:0940-9602
1618-0402
DOI:10.1016/j.aanat.2010.05.004