Pyridine Nucleotide Requirements of Fatty Acid Synthetases

The multienzyme fatty acid synthetase complexes from Mycobacterium phlei, Euglena gracilis , and Saccharomyces cerevisiae require both TPNH and DPNH for optimal efficiency. In the presence of both nucleotides, the K m values for TPNH and for DPNH for all three synthetase complexes are less than 5 µ...

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Bibliographic Details
Published in:The Journal of biological chemistry 1971-08, Vol.246 (15), p.4751-4754
Main Authors: White, 3rd, H B, Mitsuhashi, O, Bloch, K
Format: Article
Language:English
Subjects:
Acetates
Anhydrides
Animals
Carbon Isotopes
Coenzyme A
Euglena - enzymology
Fatty Acids - biosynthesis
Kinetics
Lactones - chemical synthesis
Ligases
Liver - enzymology
Macromolecular Substances
Malonates
Models, Biological
Mycobacterium - enzymology
NAD
NADP
Oxidoreductases
Polysaccharides
Rats
Saccharomyces - enzymology
Tritium
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Summary:The multienzyme fatty acid synthetase complexes from Mycobacterium phlei, Euglena gracilis , and Saccharomyces cerevisiae require both TPNH and DPNH for optimal efficiency. In the presence of both nucleotides, the K m values for TPNH and for DPNH for all three synthetase complexes are less than 5 µ m . By measuring the formation of triacetic acid lactone in the presence of either DPNH or TPNH, it has been possible to show that in M. phlei TPNH is the specific coenzyme for the β-ketoacyl reductase step while DPNH is preferentially utilized in α,β-enoyl reduction.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)61999-4