Stereospecificity of peptidyl dipeptide hydrolase (Angiotensin I-converting enzyme) [swine]

The stereospecificity of peptidyl dipeptide hydrolase [EC 3.4.15.1] was investigated. Six free and N-blocked alanyl peptides containing D-alanine were synthesized and tested as substrates. Their susceptibilities were determined by measuring Ala-Ala release by cation exchange column chromatography. T...

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Published in:Journal of biochemistry (Tokyo) 1979-12, Vol.86 (6), p.1719-1724
Main Authors: OSHIMA, Genichiro, NAGASAWA, Kinzo
Format: Article
Language:English
Subjects:
Animals
Kidney - enzymology
Kinetics
Peptidyl-Dipeptidase A - metabolism
Protein Binding
Stereoisomerism
Substrate Specificity
Swine
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container_issue 6
container_start_page 1719
container_title Journal of biochemistry (Tokyo)
container_volume 86
creator OSHIMA, Genichiro
NAGASAWA, Kinzo
description The stereospecificity of peptidyl dipeptide hydrolase [EC 3.4.15.1] was investigated. Six free and N-blocked alanyl peptides containing D-alanine were synthesized and tested as substrates. Their susceptibilities were determined by measuring Ala-Ala release by cation exchange column chromatography. Their Michaelis constants, Km values, and velocity maxima, Vmax values, were also determined. The enzyme showed high stereospecificity for an amino acyl residue in position 3 from C-terminus: it had an absolute requirement for the alanyl residue of the L-configuration in this position. An alanyl residue of the L-configuration in position 1 or 2 increased, but was not essential for activity. The enzyme showed little stereospecificity for an alanyl residue in position 4 from the C-terminus.
doi_str_mv 10.1093/oxfordjournals.jbchem.a132692
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source J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - English; Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025
subjects Animals
Kidney - enzymology
Kinetics
Peptidyl-Dipeptidase A - metabolism
Protein Binding
Stereoisomerism
Substrate Specificity
Swine
title Stereospecificity of peptidyl dipeptide hydrolase (Angiotensin I-converting enzyme) [swine]
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