Amino acid sequence of the membranous segment of rabbit liver cytochrome b5. Methodology for separation of hydrophobic peptides

The primary structure of the membranous segment of rabbit liver cytochrome b5 has been determined to be as follows: (Formula: see text), New methods were developed for the separation of peptide fragments. These methods, which are particularly useful for work with hydrophobic peptides, involved gel p...

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Bibliographic Details
Published in:The Journal of biological chemistry 1980-02, Vol.255 (4), p.1536-1541
Main Authors: Takagaki, Y, Gerber, G E, Nihei, K, Khorana, H G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - analysis
Animals
Cell Membrane - analysis
Chromatography, Gel - methods
Chromatography, High Pressure Liquid - methods
Cyanogen Bromide
Cytochromes - isolation & purification
Female
Liver - analysis
Peptide Fragments - analysis
Pregnancy
Rats
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Summary:The primary structure of the membranous segment of rabbit liver cytochrome b5 has been determined to be as follows: (Formula: see text), New methods were developed for the separation of peptide fragments. These methods, which are particularly useful for work with hydrophobic peptides, involved gel permeation chromatography and reverse phase high performance liquid chromatography (HPLC) using ethanol/formic acid mixtures. Sequences of the purified fragments were determined by automated sequence analysis.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)86065-9