Immunochemical and Biological Properties of the Outer Membrane-Associated Lipopolysaccharide and Protein of Rochalimaea quintana

The outer membrane complex of Rochalimaea quintana was isolated by use of ethylenediaminetetraacetate and was compared biochemically and biologically both with lipopolysaccharide (LPS) isolated by phenol-water extraction of whole organisms and with lipids isolated by chloroform-methanol extraction o...

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Bibliographic Details
Published in:The Journal of infectious diseases 1980-05, Vol.141 (5), p.672-679
Main Authors: Hollingdale, Michael R., Vinson, J. William, Herrmann, John E.
Format: Article
Language:English
Subjects:
Amino sugars
Animals
Bacterial Proteins
Bartonella quintana
Biochemistry
Cell Membrane - analysis
Cell Membrane - microbiology
Chick Embryo
Complement System Proteins - immunology
Deoxy Sugars
Edetic Acid - pharmacology
Embryos
Guinea Pigs
Heptoses
Horses
Immunodiffusion
Laboratory Investigation
Limulus Test
Lipids
Lipopolysaccharides
Phenols
Phenols - pharmacology
Pronase - pharmacology
Rabbits
Rickettsia - immunology
Trench fever
Trench Fever - immunology
Typhus
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Summary:The outer membrane complex of Rochalimaea quintana was isolated by use of ethylenediaminetetraacetate and was compared biochemically and biologically both with lipopolysaccharide (LPS) isolated by phenol-water extraction of whole organisms and with lipids isolated by chloroform-methanol extraction of the phenol-water insoluble residues. The outer membrane consisted of protein and LPS components, as distinguished by precipitin tests with sera from patients with trench fever or tests with hyperimmune animal sera. The outer membrane protein component, but not LPS, also reacted with sera from infections with Rickettsia tsutsugamushi. The LPS contained 2-keto-3-deoxyoctonate and heptose. The outer membrane and phenol-extracted LPS were reactive in the chick embryo lethality test, limulus assay, and complement activation. Outermembrane activity was confined to the LPS component. The lipid extracts were reactive in chick embryo lethality and limulus assays, but did not activate complement.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/141.5.672