Transient, Heat-Induced Thermal Resistance in the Small Intestine of Mouse

• Published in: Radiation research 1980-06, Vol.82 (3), p.526-535
• Main Authors: Hume, Susan P., Julia C. L. Marigold
• Format: Article
• Language: English
• Subjects: Animals; Cell Survival; CHO cells; Crypts; Female; Heat tolerance; Heat treatment; HeLa cells; Hot Temperature; Hyperthermia; Intestine, Small - pathology; Intestines; Jejunum; Mice; Radiotherapy - methods; Regeneration; Regression Analysis; Resistance heating; Space life sciences; Temperature resistance; Thermal resistance; Time Factors
• ISSN: 0033-7587; 1938-5404
• DOI: 10.2307/3575319

Heat-induced thermal resistance has been investigated in mouse jejunum by assaying crypt survival 24 hr after treatment. Hyperthermia was achieved by immersing an exteriorized loop of intestine in a bath of Krebs-Ringer solution. Two approaches have been used. In the first, thermal survival curves were obtained following single hyperthermal treatments at temperatures in the range 42-44°C. Transient thermal resistance, indicated by a plateau in the crypt survival curve, developed during heating at temperatures around 42.5°C after 60-80 min. In the second series of experiments, a priming heat treatment (40.0, 41.0, 41.5, or 42.0°C for 60 min) was followed at varying intervals by a test treatment at 43.0°C. A transient resistance to the second treatment was induced, the extent and time of development being dependent upon the priming treatment. Crypt survival curves for thermally resistant intestine showed an increase in thermal D0 and a decrease in n compared with curves from previously unheated intestine.