Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII

Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximatel...

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Bibliographic Details
Published in:Chromosoma 1980-02, Vol.77 (2), p.217-227
Main Authors: Swanton, M T, Heumann, J M, Prescott, D M
Format: Article
Language:English
Subjects:
Animals
Ciliophora - genetics
DNA - analysis
DNA, Single-Stranded - analysis
Electrophoresis, Agar Gel
Genes
Microscopy, Electron
Molecular Weight
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Summary:Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximately 20,000 bp. By EM, the number average molecular sizes for double-stranded DNA were 2,200 bp for Oxytricha sp., 2,514 bp for Stylonychia pustulata and 18,836 bp for Euplotes aediculatus. Contrary to previous reports we present evidence that the macronuclear DNA s in each of these three organisms lack single-stranded interruptions.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00329546