Novel urinary fragments from human basement membrane collagen

Five fragments of collagen have been purified from human urine. The fragments cross-reacted with basement membrane collagen from pig kidney cortices when tested against antiserum to basement membrane collagen, type IV, from murine tumor. The concentrations of these fragments were sufficient for amin...

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Bibliographic Details
Published in:The Journal of biological chemistry 1980-10, Vol.255 (19), p.9484-9489
Main Authors: Bowman, B H, Schneider, L, Barnett, D R, Kurosky, A, Goldblum, R M
Format: Article
Language:English
Subjects:
Adolescent
Adult
Amino Acids - analysis
Animals
Basement Membrane - metabolism
Carbohydrates - analysis
Child
Collagen - urine
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Humans
Immune Sera
Kidney Cortex - analysis
Molecular Weight
Peptide Fragments - urine
Swine
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Summary:Five fragments of collagen have been purified from human urine. The fragments cross-reacted with basement membrane collagen from pig kidney cortices when tested against antiserum to basement membrane collagen, type IV, from murine tumor. The concentrations of these fragments were sufficient for amino acid analysis, immunological analysis, and in one case, preliminary analytical amino acid sequencing. Compositional analysis of the fragments indicated concentrations of hydroxylysine, hydroxyproline, and glycine that are typical of collagen. High values of hexosamines and neutral sugars were also found. An NH2-terminal sequence of 18 residues was obtained for one fragment of Mr approximately 6,500. This sequence was unique among proteins reported to date, but was obviously collagen-like. A urinary source of basement membrane collagen fragments will permit molecular characterization of basement membrane in inherited or acquired diseases affecting this structure.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)70588-2