Rheumatoid Factor in Acute Viral Infections: Interference with Determination of IgM, IgG, and IgA Antibodies in an Enzyme Immunoassay

IgM rheumatoid factor (RF), an autoantibody to the Fe fragment of IgG, was determined by solid-phase enzyme immunoassay (EIA). RF levels were significantly higher in patients with rubella virus infection than in patients with infections due to influenza virus, cytomegalovirus, respiratory syncytial...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1980-08, Vol.142 (2), p.250-255
Main Authors: Salonen, Eeva-Marjatta, Vaheri, Antti, Suni, Jukka, Wager, Odd
Format: Article
Language:English
Subjects:
Acute Disease
Antibodies
Antigens
Autoantibodies - biosynthesis
Cytomegalovirus
Cytomegalovirus - immunology
Humans
Immunoenzyme Techniques
Immunoglobulin A - biosynthesis
Immunoglobulin G - biosynthesis
Immunoglobulin M - biosynthesis
Infections
Influenza, Human - immunology
Laboratory Investigation
Orthomyxoviridae
Rheumatoid Factor - immunology
Rheumatoid Factor - pharmacology
Rubella
Rubella virus
Rubella virus - immunology
Viral antibodies
Virus Diseases - immunology
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:IgM rheumatoid factor (RF), an autoantibody to the Fe fragment of IgG, was determined by solid-phase enzyme immunoassay (EIA). RF levels were significantly higher in patients with rubella virus infection than in patients with infections due to influenza virus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, adenovirus, mumps virus, or herpes simplex virus. To evaluate the role of RF in EIA determinations of viral antibodies, IgM RF from IgM-IgG cryoglobulin or control IgM was added to patient sera before assay for viral antibodies. IgM RF inhibited virus-specific IgG and IgA reactions and gave nonspecific IgM reactions in EIA for antibodies to rubella and influenza viruses, but had little or no effect on antibodies to cytomegalovirus. The minimal effective amounts of RF were 100–500 ng/ml for inhibition of IgG, 300–1,000 ng/ml for IgA, and 25–500 ng/ml for IgM. The control IgM preparation gave no such effects. These studies reinforce the need to eliminate RF interference in solid-phase EIA.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/142.2.250