Production, expansion, and clonal analysis of T cells with specific HLA-restricted male lysis

A cytotoxic T cell (CT) lines grown as a population (CT line) was initiated from the peripheral blood lympocytes (PBL) of a female aplastic anemia patient who was known to express CT that were able to lyse HLA-A2-positive male cells. The anti-H-Y HLA-A2-restricted cytotoxic activity could be maintai...

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Bibliographic Details
Published in:The Journal of experimental medicine 1980-01, Vol.152 (2 Pt 2), p.182s-190s
Main Authors: Goulmy, E, Blokland, E, van Rood, J, Charmot, D, Malissen, B, Mawas, C
Format: Article
Language:English
Subjects:
Anemia, Aplastic - immunology
Cell Membrane - immunology
Cytotoxicity, Immunologic
Female
H-Y Antigen - immunology
Histocompatibility Antigens Class II - genetics
HLA Antigens - immunology
Humans
T-Lymphocytes - immunology
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Summary:A cytotoxic T cell (CT) lines grown as a population (CT line) was initiated from the peripheral blood lympocytes (PBL) of a female aplastic anemia patient who was known to express CT that were able to lyse HLA-A2-positive male cells. The anti-H-Y HLA-A2-restricted cytotoxic activity could be maintained over prolonged periods of time. The CT lines could be expanded and maintained in culture for >65 d by the use of mitogens and irradiated feeder cells. Out of 68 cultures obtained after cloning of the CT lines, 43 showed varying, but always specific, anti-H-Y HLA-A2-restricted lytic capacity on a per-cell basis. We could show that the cloned cultures were composed of >80% T cells that carry the HLA-A, -B, -C, and also the HLA-DR antigens identical to the original PBL.
ISSN:0022-1007