The Isolation and Characterization of a Clonally Related Series of Murine Retrovirus-infected Mouse Cells

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K. Starting with cloned NIH 3T3 mouse cells we have isolated a series of related lines infected with the Kirsten murine sarcoma/leukaemia (MSV/MLV) virus complex. These lines exhibit all three possible infected cell phenot...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 1980-07, Vol.49 (1), p.105-113
Main Authors: Morris, Alan, Clegg, Chris, Jones, Jenny, Rodgers, Brian, Avery, Roger J
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
Cell Line
Cell Transformation, Neoplastic
Cell Transformation, Viral
Clone Cells - microbiology
Fibroblasts
Kirsten murine sarcoma virus - physiology
Leukemia Virus, Murine - growth & development
Mice
Sarcoma Viruses, Murine - growth & development
Sarcoma Viruses, Murine - physiology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K. Starting with cloned NIH 3T3 mouse cells we have isolated a series of related lines infected with the Kirsten murine sarcoma/leukaemia (MSV/MLV) virus complex. These lines exhibit all three possible infected cell phenotopes: (i) transformed MSV/MLV producers; (ii) non-transformed MLV producers; (iii) transformed non-producers. We have also selected non-transformed revertants from one of the non-producer clones. This series of lines allows the study of the expression of the virus genome against a constant background of cellular gene expression. We have further characterized the lines with regard to anchorage dependence of growth, tumorigenicity and the presence of a rescuable sarcoma genome. The non-producer clones are uniform in their transformed properties. The revertants contain rescuable sarcoma virus, biologically indistinguishable from the original transforming virus, implying that the reversion is due to a change in cellular rather than virus genetic information. * Present address: Pharmakologisches Institut der Universitat Mainz, D-6500 Mainz, F.R.G. Present address: Department of Microbiology, Guy's Hospital Medical School, London Bridge, London SE1 9RT, U.K. Received 25 October 1979; accepted 12 February 1980.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-49-1-105