Large Scale Production of Mouse Interferons from Monolayers of Ehrlich Ascites Tumour Cells

Department of Molecular Biophysics and Biochemistry Yale University, New Haven Connecticut 06511, U.S.A. Conditions are described for the large scale production of mouse interferons from Ehrlich ascites tumour cells cultured as monolayers in roller bottles. With the procedure reported here, we have...

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Bibliographic Details
Published in:Journal of general virology 1980-07, Vol.49 (1), p.231-234
Main Authors: Taira, H, Broeze, R. J, Slattery, E, Lengyel, P
Format: Article
Language:English
Subjects:
Animals
Blood
Carcinoma, Ehrlich Tumor
Cell Line
Culture Media
Interferons - biosynthesis
Interferons - isolation & purification
Methods
Mice
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Summary:Department of Molecular Biophysics and Biochemistry Yale University, New Haven Connecticut 06511, U.S.A. Conditions are described for the large scale production of mouse interferons from Ehrlich ascites tumour cells cultured as monolayers in roller bottles. With the procedure reported here, we have used 50 to 65 600 cm 2 roller bottles to produce routinely 2 x 10 9 to 3 x 10 9 International units of crude mouse interferon/week with a specific activity of 1 x 10 6 to 1.5 x 10 6 units/mg protein. Received 8 January 1980; accepted 7 February 1980.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-49-1-231