Expression of human fibroblast interferon gene in Escherichia coli

The human fibroblast Interferon gene was inserted in a thermoinducible expression plasmid under control of the phage lambda P L promotor. The primary translation products predicted on the basis of the plasmid constructions were hybrid proteins starting with β-lactamase or phage MS2 polymerase inform...

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Bibliographic Details
Published in:Nature (London) 1980-09, Vol.287 (5779), p.193-197
Main Authors: Derynck, Rik, Remaut, Erik, Saman, Erik, Stanssens, Patrick, Clercq, Erik De, Content, Jean, Fiers, Walter
Format: Article
Language:English
Subjects:
Biological Assay
DNA, Recombinant
Escherichia coli - genetics
Fibroblasts - physiology
Gene Expression Regulation
Humanities and Social Sciences
Humans
Interferons - genetics
Molecular Weight
multidisciplinary
Plasmids
Science
Science (multidisciplinary)
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Summary:The human fibroblast Interferon gene was inserted in a thermoinducible expression plasmid under control of the phage lambda P L promotor. The primary translation products predicted on the basis of the plasmid constructions were hybrid proteins starting with β-lactamase or phage MS2 polymerase information followed by the total preinterferon. On induction, antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized. Processing to a size compatible with mature but unglycosylated authentic product was observed.
ISSN:0028-0836
1476-4687
DOI:10.1038/287193a0