Loading…

Insulin binding and insulin action in rat fat cells after adrenalectomy

Insulin binding and the effect of insulin on the transport of 3-O-methylglucose, lipogenesis from glucose, glucose oxidation and lipolysis was studied in fat cells of adrenalectomised rats and of a control group of sham-operated rats. The serum insulin level of the adrenalectomised rats (0.7 ng/ml)...

Full description

Saved in:
Bibliographic Details
Published in:Diabetologia 1980-10, Vol.19 (4), p.379-385
Main Authors: Häring, H, Calle, C, Bug, A, Renner, R, Hepp, K D, Kemmler, W
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c318t-830afdef169c36e8a337242be61ce4b15cf8b8d372f37d7deed8a64829ad121a3
cites cdi_FETCH-LOGICAL-c318t-830afdef169c36e8a337242be61ce4b15cf8b8d372f37d7deed8a64829ad121a3
container_end_page 385
container_issue 4
container_start_page 379
container_title Diabetologia
container_volume 19
creator Häring, H
Calle, C
Bug, A
Renner, R
Hepp, K D
Kemmler, W
description Insulin binding and the effect of insulin on the transport of 3-O-methylglucose, lipogenesis from glucose, glucose oxidation and lipolysis was studied in fat cells of adrenalectomised rats and of a control group of sham-operated rats. The serum insulin level of the adrenalectomised rats (0.7 ng/ml) was lower than that of the controls (1.6 ng/ml). In adrenalectomised rats as compared to sham-operated rats the insulin concentrations causing half-maximal effect were reduced by 50% in lipogenesis and antilipolysis and by 30% in glucose transport. The increase in sensitivity to submaximal insulin concentrations was not observed in glucose oxidation. The maximal responsiveness was unchanged in all test systems. The increase in sensitivity in three of the four studied insulin effects may be related to the 37% increase in the binding capacity of fat cells from adrenalectomised compared with sham-operated rats. The unchanged sensitivity with respect to glucose oxidation indicates possible post-receptor modulation. When adrenalectomised rats were substituted with either insulin or cortisol serum insulin levels were elevated above normal; however, the changes in the receptor were prevented in the cortisol supplemented rats and only partially in the insulin supplemented rats. The observation suggests, that the insulin receptor is regulated not only by the serum insulin level but also by cortisol.
doi_str_mv 10.1007/BF00280524
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75319358</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75319358</sourcerecordid><originalsourceid>FETCH-LOGICAL-c318t-830afdef169c36e8a337242be61ce4b15cf8b8d372f37d7deed8a64829ad121a3</originalsourceid><addsrcrecordid>eNpFkM1LxDAQxYMo67p68S7k5EGoTj6aZo-6uOvCghcFbyVNJlJp0zVpD_vfW9mih2FmHj8ej0fINYN7BlA8PK0BuIacyxMyZ1LwDCTXp2QOwHjGtPo4JxcpfQGAyKWakVkxngpgTjbbkIamDrSqg6vDJzXB0XrSjO3rLowvjaanfhyLTZOo8T1GalzEYBq0fdceLsmZN03Cq2kvyPv6-W31ku1eN9vV4y6zguk-0wKMd-iZWlqhUBshCi55hYpZlBXLrdeVdqPoReEKh-i0UVLzpXGMMyMW5Pbou4_d94CpL9s6_aYyAbshlUUu2FLkegTvjqCNXUoRfbmPdWvioWRQ_rZW_rc2wjeT61C16P7QqSbxA2e3Zuc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75319358</pqid></control><display><type>article</type><title>Insulin binding and insulin action in rat fat cells after adrenalectomy</title><source>Springer Online Journal Archives (Through 1996)</source><creator>Häring, H ; Calle, C ; Bug, A ; Renner, R ; Hepp, K D ; Kemmler, W</creator><creatorcontrib>Häring, H ; Calle, C ; Bug, A ; Renner, R ; Hepp, K D ; Kemmler, W</creatorcontrib><description>Insulin binding and the effect of insulin on the transport of 3-O-methylglucose, lipogenesis from glucose, glucose oxidation and lipolysis was studied in fat cells of adrenalectomised rats and of a control group of sham-operated rats. The serum insulin level of the adrenalectomised rats (0.7 ng/ml) was lower than that of the controls (1.6 ng/ml). In adrenalectomised rats as compared to sham-operated rats the insulin concentrations causing half-maximal effect were reduced by 50% in lipogenesis and antilipolysis and by 30% in glucose transport. The increase in sensitivity to submaximal insulin concentrations was not observed in glucose oxidation. The maximal responsiveness was unchanged in all test systems. The increase in sensitivity in three of the four studied insulin effects may be related to the 37% increase in the binding capacity of fat cells from adrenalectomised compared with sham-operated rats. The unchanged sensitivity with respect to glucose oxidation indicates possible post-receptor modulation. When adrenalectomised rats were substituted with either insulin or cortisol serum insulin levels were elevated above normal; however, the changes in the receptor were prevented in the cortisol supplemented rats and only partially in the insulin supplemented rats. The observation suggests, that the insulin receptor is regulated not only by the serum insulin level but also by cortisol.</description><identifier>ISSN: 0012-186X</identifier><identifier>EISSN: 1432-0428</identifier><identifier>DOI: 10.1007/BF00280524</identifier><identifier>PMID: 7000600</identifier><language>eng</language><publisher>Germany</publisher><subject>Adipose Tissue - drug effects ; Adipose Tissue - metabolism ; Adrenalectomy ; Animals ; Biological Transport, Active ; Body Weight - drug effects ; Glucose - metabolism ; Hydrocortisone - pharmacology ; Insulin - metabolism ; Insulin - pharmacology ; Kinetics ; Lipid Mobilization - drug effects ; Male ; Methylglucosides - metabolism ; Rats ; Receptor, Insulin - metabolism</subject><ispartof>Diabetologia, 1980-10, Vol.19 (4), p.379-385</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c318t-830afdef169c36e8a337242be61ce4b15cf8b8d372f37d7deed8a64829ad121a3</citedby><cites>FETCH-LOGICAL-c318t-830afdef169c36e8a337242be61ce4b15cf8b8d372f37d7deed8a64829ad121a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7000600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Häring, H</creatorcontrib><creatorcontrib>Calle, C</creatorcontrib><creatorcontrib>Bug, A</creatorcontrib><creatorcontrib>Renner, R</creatorcontrib><creatorcontrib>Hepp, K D</creatorcontrib><creatorcontrib>Kemmler, W</creatorcontrib><title>Insulin binding and insulin action in rat fat cells after adrenalectomy</title><title>Diabetologia</title><addtitle>Diabetologia</addtitle><description>Insulin binding and the effect of insulin on the transport of 3-O-methylglucose, lipogenesis from glucose, glucose oxidation and lipolysis was studied in fat cells of adrenalectomised rats and of a control group of sham-operated rats. The serum insulin level of the adrenalectomised rats (0.7 ng/ml) was lower than that of the controls (1.6 ng/ml). In adrenalectomised rats as compared to sham-operated rats the insulin concentrations causing half-maximal effect were reduced by 50% in lipogenesis and antilipolysis and by 30% in glucose transport. The increase in sensitivity to submaximal insulin concentrations was not observed in glucose oxidation. The maximal responsiveness was unchanged in all test systems. The increase in sensitivity in three of the four studied insulin effects may be related to the 37% increase in the binding capacity of fat cells from adrenalectomised compared with sham-operated rats. The unchanged sensitivity with respect to glucose oxidation indicates possible post-receptor modulation. When adrenalectomised rats were substituted with either insulin or cortisol serum insulin levels were elevated above normal; however, the changes in the receptor were prevented in the cortisol supplemented rats and only partially in the insulin supplemented rats. The observation suggests, that the insulin receptor is regulated not only by the serum insulin level but also by cortisol.</description><subject>Adipose Tissue - drug effects</subject><subject>Adipose Tissue - metabolism</subject><subject>Adrenalectomy</subject><subject>Animals</subject><subject>Biological Transport, Active</subject><subject>Body Weight - drug effects</subject><subject>Glucose - metabolism</subject><subject>Hydrocortisone - pharmacology</subject><subject>Insulin - metabolism</subject><subject>Insulin - pharmacology</subject><subject>Kinetics</subject><subject>Lipid Mobilization - drug effects</subject><subject>Male</subject><subject>Methylglucosides - metabolism</subject><subject>Rats</subject><subject>Receptor, Insulin - metabolism</subject><issn>0012-186X</issn><issn>1432-0428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><recordid>eNpFkM1LxDAQxYMo67p68S7k5EGoTj6aZo-6uOvCghcFbyVNJlJp0zVpD_vfW9mih2FmHj8ej0fINYN7BlA8PK0BuIacyxMyZ1LwDCTXp2QOwHjGtPo4JxcpfQGAyKWakVkxngpgTjbbkIamDrSqg6vDJzXB0XrSjO3rLowvjaanfhyLTZOo8T1GalzEYBq0fdceLsmZN03Cq2kvyPv6-W31ku1eN9vV4y6zguk-0wKMd-iZWlqhUBshCi55hYpZlBXLrdeVdqPoReEKh-i0UVLzpXGMMyMW5Pbou4_d94CpL9s6_aYyAbshlUUu2FLkegTvjqCNXUoRfbmPdWvioWRQ_rZW_rc2wjeT61C16P7QqSbxA2e3Zuc</recordid><startdate>198010</startdate><enddate>198010</enddate><creator>Häring, H</creator><creator>Calle, C</creator><creator>Bug, A</creator><creator>Renner, R</creator><creator>Hepp, K D</creator><creator>Kemmler, W</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198010</creationdate><title>Insulin binding and insulin action in rat fat cells after adrenalectomy</title><author>Häring, H ; Calle, C ; Bug, A ; Renner, R ; Hepp, K D ; Kemmler, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c318t-830afdef169c36e8a337242be61ce4b15cf8b8d372f37d7deed8a64829ad121a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Adipose Tissue - drug effects</topic><topic>Adipose Tissue - metabolism</topic><topic>Adrenalectomy</topic><topic>Animals</topic><topic>Biological Transport, Active</topic><topic>Body Weight - drug effects</topic><topic>Glucose - metabolism</topic><topic>Hydrocortisone - pharmacology</topic><topic>Insulin - metabolism</topic><topic>Insulin - pharmacology</topic><topic>Kinetics</topic><topic>Lipid Mobilization - drug effects</topic><topic>Male</topic><topic>Methylglucosides - metabolism</topic><topic>Rats</topic><topic>Receptor, Insulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Häring, H</creatorcontrib><creatorcontrib>Calle, C</creatorcontrib><creatorcontrib>Bug, A</creatorcontrib><creatorcontrib>Renner, R</creatorcontrib><creatorcontrib>Hepp, K D</creatorcontrib><creatorcontrib>Kemmler, W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diabetologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Häring, H</au><au>Calle, C</au><au>Bug, A</au><au>Renner, R</au><au>Hepp, K D</au><au>Kemmler, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin binding and insulin action in rat fat cells after adrenalectomy</atitle><jtitle>Diabetologia</jtitle><addtitle>Diabetologia</addtitle><date>1980-10</date><risdate>1980</risdate><volume>19</volume><issue>4</issue><spage>379</spage><epage>385</epage><pages>379-385</pages><issn>0012-186X</issn><eissn>1432-0428</eissn><abstract>Insulin binding and the effect of insulin on the transport of 3-O-methylglucose, lipogenesis from glucose, glucose oxidation and lipolysis was studied in fat cells of adrenalectomised rats and of a control group of sham-operated rats. The serum insulin level of the adrenalectomised rats (0.7 ng/ml) was lower than that of the controls (1.6 ng/ml). In adrenalectomised rats as compared to sham-operated rats the insulin concentrations causing half-maximal effect were reduced by 50% in lipogenesis and antilipolysis and by 30% in glucose transport. The increase in sensitivity to submaximal insulin concentrations was not observed in glucose oxidation. The maximal responsiveness was unchanged in all test systems. The increase in sensitivity in three of the four studied insulin effects may be related to the 37% increase in the binding capacity of fat cells from adrenalectomised compared with sham-operated rats. The unchanged sensitivity with respect to glucose oxidation indicates possible post-receptor modulation. When adrenalectomised rats were substituted with either insulin or cortisol serum insulin levels were elevated above normal; however, the changes in the receptor were prevented in the cortisol supplemented rats and only partially in the insulin supplemented rats. The observation suggests, that the insulin receptor is regulated not only by the serum insulin level but also by cortisol.</abstract><cop>Germany</cop><pmid>7000600</pmid><doi>10.1007/BF00280524</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0012-186X
ispartof Diabetologia, 1980-10, Vol.19 (4), p.379-385
issn 0012-186X
1432-0428
language eng
recordid cdi_proquest_miscellaneous_75319358
source Springer Online Journal Archives (Through 1996)
subjects Adipose Tissue - drug effects
Adipose Tissue - metabolism
Adrenalectomy
Animals
Biological Transport, Active
Body Weight - drug effects
Glucose - metabolism
Hydrocortisone - pharmacology
Insulin - metabolism
Insulin - pharmacology
Kinetics
Lipid Mobilization - drug effects
Male
Methylglucosides - metabolism
Rats
Receptor, Insulin - metabolism
title Insulin binding and insulin action in rat fat cells after adrenalectomy
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T04%3A45%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Insulin%20binding%20and%20insulin%20action%20in%20rat%20fat%20cells%20after%20adrenalectomy&rft.jtitle=Diabetologia&rft.au=H%C3%A4ring,%20H&rft.date=1980-10&rft.volume=19&rft.issue=4&rft.spage=379&rft.epage=385&rft.pages=379-385&rft.issn=0012-186X&rft.eissn=1432-0428&rft_id=info:doi/10.1007/BF00280524&rft_dat=%3Cproquest_cross%3E75319358%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c318t-830afdef169c36e8a337242be61ce4b15cf8b8d372f37d7deed8a64829ad121a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=75319358&rft_id=info:pmid/7000600&rfr_iscdi=true