Paper Chromatography of Amino-Acids in Cerebrospinal Fluid

WE have been investigating the amino-acids present in the cerebrospinal fluid obtained from patients with various diseases, using paper chromatography. Successful chromatograms require that protein and electrolytes should be removed from the fluid. There have been various methods suggested for doing...

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Bibliographic Details
Published in:Nature (London) 1953-08, Vol.172 (4373), p.358-359
Main Authors: PRIOR, A. P, WHITEHEAD, T. P
Format: Article
Language:English
Subjects:
Amino Acids - cerebrospinal fluid
Cerebrospinal Fluid
Chromatography
Chromatography, Paper
Humanities and Social Sciences
letter
multidisciplinary
Old Medline
Science
Science (multidisciplinary)
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Summary:WE have been investigating the amino-acids present in the cerebrospinal fluid obtained from patients with various diseases, using paper chromatography. Successful chromatograms require that protein and electrolytes should be removed from the fluid. There have been various methods suggested for doing this. We use a method which is simple, quick and requires apparatus that is readily available. It is based on the work of Boulanger and Biserte 1 , who showed that, when biological fluids containing amino-acids are passed through cation-exchange resins, all the amino-acids with the exception of taurine are retained in the resin along with the cations, and that the anions pass through. The amino-acids can then be displaced from the resin with ammonia, leaving the cations on the column.
ISSN:0028-0836
1476-4687
DOI:10.1038/172358a0