THE MECHANISM OF ACTION OF POLYCHLOROSUBTILIN

Polychlorosubtilin (PCS) inhibits the growth of Escherichia coli. The antibiotic affected neither respiration nor glycolysis while the synthesis of nucleic acids and proteins were feebly hindered. Formation of aminoacyl-tRNA, peptide bonds and translocation from A to P sites of ribosomes were insign...

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Bibliographic Details
Published in:Journal of antibiotics 1980, Vol.33(9), pp.1063-1070
Main Authors: SHIMI, IBRAHIM R., ALI, FAHMY T.
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Anti-Bacterial Agents - pharmacology
Bacterial Proteins - biosynthesis
DNA, Bacterial - biosynthesis
Escherichia coli - drug effects
Escherichia coli - growth & development
Escherichia coli - metabolism
Peptides
Peptides, Cyclic - pharmacology
RNA, Bacterial - biosynthesis
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Summary:Polychlorosubtilin (PCS) inhibits the growth of Escherichia coli. The antibiotic affected neither respiration nor glycolysis while the synthesis of nucleic acids and proteins were feebly hindered. Formation of aminoacyl-tRNA, peptide bonds and translocation from A to P sites of ribosomes were insignificantly influenced by the drug. The antibiotic exerted its effect(s) on ribosomes by interfering with the 30 S subunits. The 23 S and 30 SP were both sensitive to the drug but the latter was more obviously affected. Changes after developing resistance to the drug by the bacteria were localized in the 30 SP, 23 S and accordingly the 30 S subunits. The principal action of PCS was to cause multisited miscoding upon the incorporation of labeled aminoacyl-tRNA, therefore, malformed protein fractions (abnormal) were synthesized. As a natural consequence such abnormal fractions would not be expected to manifest the vital metabolic activities in the normal way.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.33.1063