Calcitonin and 1,25-dihydroxyvitamin D3 receptors in human breast cancer cell lines

• Published in: Cancer research (Chicago, Ill.) Ill.), 1980-12, Vol.40 (12), p.4764-4767
• Main Authors: Findlay, D M, Michelangeli, V P, Eisman, J A, Frampton, R J, Moseley, J M, MacIntyre, I, Whitehead, R, Martin, T J
• Format: Article
• Language: English
• Subjects: Adenylyl Cyclases - metabolism; Breast Neoplasms - metabolism; Calcitonin - metabolism; Calcitonin - pharmacology; Cell Line; Dihydroxycholecalciferols - metabolism; Enzyme Activation - drug effects; Female; Humans; Hydroxycholecalciferols - metabolism; Receptors, Calcitonin; Receptors, Calcitriol; Receptors, Cell Surface - metabolism; Receptors, Steroid - metabolism; Structure-Activity Relationship
• ISSN: 0008-5472

Five human breast cancer cell lines (MCF 7, T 47D, BT 20, MDA 157, and MDA 231) and a human breast epithelial cell line (HBL 100) have been found to contain specific high-affinity receptors for 1,25-dihydroxyvitamin D3, Kd values ranged from 0.6 to 2.0 X 10(-11) M and receptor concentration from 31 to 150 fmol/mg cytosol protein. Two of the breast cancer lines (MCF 7 and T 47D) contain specific high-affinity receptors for calcitonin and a calcitonin-responsive adenylate cyclase, which have been characterized with the aid of salmon, eel, and human calcitonins and in several substituted analogues of human calcitonin. The 1,25-dihydroxyvitamin D3 receptor may reflect a normal property of the breast cell. Breast cancer cell lines provide a useful source of 1,25-dihydroxyvitamin D3 receptors. Their coexistence with a calcitonin receptor and biological response in some breast cancers offers the opportunity to investigate new aspects of breast cancer endocrinology.