Principles of responsive lanthanide-based luminescent probes for cellular imaging

The advent of chemical tools for cellular imaging--from organic dyes to green fluorescent proteins--has revolutionized the fields of molecular biology and biochemistry. Lanthanide-based probes are a new player in this area, as the last decade has seen the emergence of the first responsive luminescen...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2009-05, Vol.394 (1), p.107-120
Main Authors: Thibon, Aurore, Pierre, Valérie C
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antennas
Biochemistry
Cells - cytology
Cellular
Cellular probes
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Dyes
Emergence
Food Science
Imaging
Laboratory Medicine
Lanthanide luminescence
Lanthanides
Lanthanoid Series Elements - chemistry
Luminescence
Luminescent Measurements
Luminescent Proteins - chemistry
Molecular Probe Techniques
Molecular Probes - analysis
Molecular Probes - chemistry
Monitoring/Environmental Analysis
Photoelectron transfer
Review
Sensitized emission
Sensors
Time-gated detection
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Summary:The advent of chemical tools for cellular imaging--from organic dyes to green fluorescent proteins--has revolutionized the fields of molecular biology and biochemistry. Lanthanide-based probes are a new player in this area, as the last decade has seen the emergence of the first responsive luminescent lanthanide probes specifically intended for imaging cellular processes. The potential of these probes is still undervalued by the scientific community. Indeed, this class of probes offers several advantages over organic dyes and fluorescent proteins. Their very long luminescence lifetimes enable quantitative spatial determination of the intracellular concentration of an analyte through time-gating measurements. Their emission bands are very narrow and do not overlap, enabling the simultaneous use of multiple lanthanide probes to quantitatively detect several analytes without cross-interference. Herein we describe the principles behind the development of this class of probes. Sensors for a desired analyte can be designed by rationally manipulating the parameters that influence the luminescence of lanthanide complexes. We will discuss sensors based on varying the number of inner-sphere water molecules, the distance separating the antenna from the lanthanide ion, the energies of excited states of the antenna, and PeT switches.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-009-2683-2