Quantitative protein expression profiling of 14-3-3 isoforms in human renal carcinoma shows 14-3-3 epsilon is involved in limitedly increasing renal cell proliferation

14-3-3 proteins regulate many cellular processes that are implicated in cancer development, and the seven 14-3-3 isoforms have different expression level and isoform-specific roles in different tumors. However, the biological functions of 14-3-3 proteins and their correlations with renal carcinoma h...

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Bibliographic Details
Published in:Electrophoresis 2009-12, Vol.30 (23), p.4152-4162
Main Authors: Liang, Shufang, Xu, Yuhuan, Shen, Guobo, Liu, Qingping, Zhao, Xinyu, Xu, Zhizhong, Xie, Xi, Gong, Fengming, Li, Ronghui, Wei, Yuquan
Format: Article
Language:English
Subjects:
14-3-3 isoforms
14-3-3 Proteins - genetics
14-3-3 Proteins - metabolism
Amino acids
Blotting, Western
Carcinoma, Renal Cell - metabolism
Cell Line, Tumor
Cell Proliferation
Culture
Human
Humans
Immunohistochemistry
Isotope Labeling
Kidney Neoplasms - metabolism
Kidneys
Leu-d3
Profiling
Protein Isoforms
Proteins
Proteomics
Proteomics - methods
Quantitative proteomics
Renal cell carcinoma
Reverse Transcriptase Polymerase Chain Reaction
SILAC
Tumors
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Summary:14-3-3 proteins regulate many cellular processes that are implicated in cancer development, and the seven 14-3-3 isoforms have different expression level and isoform-specific roles in different tumors. However, the biological functions of 14-3-3 proteins and their correlations with renal carcinoma have not been investigated so far. In our study, the expression profiles and functional characterization of 14-3-3 proteins were discovered by a sensitive stable isotope labeling with amino acids in cell culture based quantitative proteomics analysis in human renal carcinoma tissues. We found that 14-3-3ε was up-regulated with 1.44-fold changes in renal cancerous tissues compared with that in counterpart kidney tissues, and 14-3-3σ was almost not detected in both tissues due to its DNA highly methylated in our previous reports. The other five isoforms almost have similar expression level in two states of renal tissues. The following RT-PCR, Western blot and immunohistochemistry analysis for specific 14-3-3 isoform expression were all consistent with the quantitative proteomic data. Furthermore, the overexpression of 14-3-3ε in vitro can limitedly prompt the abnormal growth of renal tumor cells.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200900249