Monitoring of vesicular exocytosis from single cells using micrometer and nanometer-sized electrochemical sensors

Communication between cells by release of specific chemical messengers via exocytosis plays crucial roles in biological process. Electrochemical detection based on ultramicroelectrodes (UMEs) has become one of the most powerful techniques in real-time monitoring of an extremely small number of relea...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2009-05, Vol.394 (1), p.17-32
Main Authors: Wang, Wei, Zhang, Shu-Hui, Li, Lin-Mei, Wang, Zong-Li, Cheng, Jie-Ke, Huang, Wei-Hua
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Biological
Cells - cytology
Cells - metabolism
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electrochemistry
Exocytosis
Extreme values
Food Science
Lab-on-a-chip
Laboratory Medicine
Mathematical analysis
Microchip Analytical Procedures
Microelectrode arrays
Microelectrodes
Micrometer and nanometer-sized electrochemical sensors
Micrometers
Microscopy
Monitoring
Monitoring/Environmental Analysis
Nanostructures - chemistry
Particle Size
Review
Scanning
Scanning electrochemical microscopy
Sensors
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Summary:Communication between cells by release of specific chemical messengers via exocytosis plays crucial roles in biological process. Electrochemical detection based on ultramicroelectrodes (UMEs) has become one of the most powerful techniques in real-time monitoring of an extremely small number of released molecules during very short time scales, owing to its intrinsic advantages such as fast response, excellent sensitivity, and high spatiotemporal resolution. Great successes have been achieved in the use of UME methods to obtain quantitative and kinetic information about released chemical messengers and to reveal the molecular mechanism in vesicular exocytosis. In this paper, we review recent developments in monitoring exocytosis by use of UMEs-electrochemical-based techniques including electrochemical detection using micrometer and nanometer-sized sensors, scanning electrochemical microscopy (SECM), and UMEs implemented in lab-on-a-chip (LOC) microsystems. These advances are of great significance in obtaining a better understanding of vesicular exocytosis and chemical communications between cells, and will facilitate developments in many fields, including analytical chemistry, biological science, and medicine. Furthermore, future developments in electrochemical probing of exocytosis are also proposed. [graphic removed]
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-009-2703-2