Daptomycin determination by liquid chromatography-mass spectrometry in peritoneal fluid, blood plasma, and urine of clinical patients receiving peritoneal dialysis treatment

• Published in: Analytical & bioanalytical chemistry (Print) 2010-07, Vol.397 (6), p.2191-2197
• Main Authors: Gika, H. G, Michopoulos, F, Divanis, D, Metalidis, S, Nikolaidis, P, Theodoridis, G. A
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacokinetics; Antibiotics; Ascitic Fluid; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - methods; daptomycin; Daptomycin - analysis; Daptomycin - blood; Daptomycin - pharmacokinetics; Daptomycin - urine; Detectors; Exact sciences and technology; Fluid dynamics; Fluid flow; Food Science; Gram-Positive Bacterial Infections - drug therapy; Humans; Laboratory Medicine; LC-MS; Liquid chromatography; Liquids; Mathematical analysis; Monitoring/Environmental Analysis; Original Paper; Other chromatographic methods; Peritoneal dialysis; Peritoneal Dialysis - methods; Peritoneal fluid; pharmacokinetics; Plasma; Receiving; Renal Replacement Therapy; Spectrometric and optical methods; Spectrometry; Spectrometry, Mass, Electrospray Ionization - methods; Urine
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-010-3639-2

A 13-min LC-MS method was developed for the determination of daptomycin, a new potent antibiotic, in peritoneal fluid, blood plasma, and urine of patients receiving renal replacement therapy. Chromatography was performed on a C₁₈ column and detection was performed by a single-quadrupole mass spectrometer coupled to LC via an electrospray interface (ESI). The column effluent was also monitored at 370 nm using a photodiode-array detector. The developed method provided a linear dynamic range for concentrations from 0.5 μg mL⁻¹ to 100 μg mL⁻¹. Method precision and accuracy were found to be satisfactory for clinical application, thus the method was successfully used for the analysis of daptomycin in pharmacokinetic studies. The drug was preventively administered against Gram-positive infections to 19 clinical patients undergoing peritoneal dialysis. Peritoneal fluid, blood plasma, and urine samples were collected at 13 time points over a period of 48 h. Clinical samples were analysed following simple sample-preparation procedures and daptomycin was unambiguously detected and quantified.