Metal-free Phtalocyanine and 5-Aminolevulenic Acid in Photodynamic Treatment of Human Vascular Cells

Originally developed as a tumor therapy, now photodynamic therapy (PDT) may become a useful tool for treatment of cardiovascular diseases. Different cell types are involved in this vascular pathology, and these cells possess different susceptibility to PDT. In this study we screened the effects of t...

Full description

Saved in:
Bibliographic Details
Published in:Selected Papers at the International Laser Medicine Congress 2009-11, Vol.1226, p.59-65
Main Authors: Udartseva, Olga O, Andreeva, Elena R, Buravkova, Ludmila B, Tararak, Eduard M
Format: Article
Language:English
Subjects:
Aorta
Cellular
Culture
Direct current
Diseases
Human
Illumination
Lasers
Macrophages
Muscles
Pathology
Photodynamic therapy
Sheet molding compounds
Therapy
Tumors
Viability
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Originally developed as a tumor therapy, now photodynamic therapy (PDT) may become a useful tool for treatment of cardiovascular diseases. Different cell types are involved in this vascular pathology, and these cells possess different susceptibility to PDT. In this study we screened the effects of two new photosensitizers (PtS and ALA) on human vascular cells. Human macrophages (Mph), aorta endothelial (HAEC) and smooth muscle (SMC) cells were obtained and cultured as described elsewhere. 2-10 ug/ml PtS was added to culture medium 24 h before PDT. ALA was added in 2-10 mM concentration in serum-free culture medium. Then cells were washed carefully and illuminated with 692-nm (PtS) or 633-nm (ALA) light Cellular viability was measured with MTT-test. Except the case of use 5-10 mM ALA, either photosensitizer accumulation alone or laser illumination alone did not affect cells. Illumination of PtS or ALA-loaded cells (1-20 J/cm(2)) impaired cellular viability in dose-dependent manner. LD(90) for different vascular cells with PtS were as follows: HAEC-1 J/cm(2), SMC-2 J/cm(2), Mph-5 J/cm(2). HAEC and some Mph were unsusceptible to ALA-PDT. SMC LD(90) with ALA was 20 J/cm(2). Effects of ALA-PDT depended on protoporphyrin DC (PpDC) formation in cells. HAEC didn't accumulate PpDC and were non-sensitive to ALA-PDT. PpK formation in Mph changed individually according to donor. Illumination of ALA-loaded Mph with low PpDC formation did not affect cells. However LD(90) for Mph with high PpDC formation comprised 20 J/cm(2). All cell types were more susceptible to PtS-PDT compared to ALA-PDT. Among tested photosensitizers PtS was the most effective one. HAEC were the most susceptible to PtS-PDT.
ISSN:0094-243X