Production and extraction optimization of xylanase from Aspergillus niger DFR-5 through solid-state-fermentation

The effects of solid substrates, initial moisture content, moistening medium, temperature and incubation time on xylanase production by Aspergillus niger DFR-5 was studied and the highest activity (2596 IU/g dry substrate (gds)) was achieved in medium that contained wheat bran (WB) and soybean cake...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology 2010-10, Vol.101 (19), p.7563-7569
Main Authors: Pal, Ajay, Khanum, Farhath
Format: Article
Language:English
Subjects:
Agriculture
Analysis of Variance
Aspergillus niger
Aspergillus niger - drug effects
Aspergillus niger - enzymology
Biological and medical sciences
Biotechnology
Biotechnology - methods
Drying
Endo-1,4-beta Xylanases - biosynthesis
Endo-1,4-beta Xylanases - isolation & purification
Extraction
Fermentation - drug effects
Fundamental and applied biological sciences. Psychology
Industry
Laboratories
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Moisture content
Optimization
Recovery
Regression Analysis
Solid-state-fermentation
Solvents - pharmacology
Soybeans
Statistical design
Temperature
Time Factors
Triticum aestivum
Waste Products - analysis
Water - chemistry
Xylanase
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effects of solid substrates, initial moisture content, moistening medium, temperature and incubation time on xylanase production by Aspergillus niger DFR-5 was studied and the highest activity (2596 IU/g dry substrate (gds)) was achieved in medium that contained wheat bran (WB) and soybean cake (SBC) at a ratio of 70:30, was moistened to 70% with MSS-2 mineral salt solution, and incubated for 6 days at 40 °C. Water at 37 °C was suitable for efficient recovery of enzyme from moldy WB–SBC medium. The extraction parameters for xylanase were optimized with respect to minimum volume of extractant using a central composite rotatable design (CCRD). The maximum recovery of xylanase (4465 ± 52 IU/gds) with 92.5% desirability was obtained employing water (10 ml/gds) as extractant at 200 rpm for 60 min. The result shows that an overall 5.4-fold increase in xylanase production was obtained in concentrated form by optimizing medium components and extraction conditions.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2010.04.033