A Novel HPLC-PDA-MS Method for S-Adenosylmethionine and S-Adenosylhomocysteine Routine Analysis

S-adenosylmethionine and S-adenosylhomocysteine are essential compounds in metabolic pathways; therefore, the development of a suitable method to routinely quantify these compounds is very important. In this paper, a simple and fast method (10 minutes) for simultaneous SAM and SAH quantification in...

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Bibliographic Details
Published in:Analytical letters 2010-01, Vol.43 (5), p.793-803
Main Authors: Birsan, Camelia, Litescu, Simona C., Radu, Gabriel L.
Format: Article
Language:English
Subjects:
Acoustic microscopes
Analytical chemistry
Blood
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Extraction
HPLC
Mathematical analysis
Other chromatographic methods
Pathways
Routines
S-adenosylhomocysteine
S-adenosylmethionine
Solid phases
SPE
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Summary:S-adenosylmethionine and S-adenosylhomocysteine are essential compounds in metabolic pathways; therefore, the development of a suitable method to routinely quantify these compounds is very important. In this paper, a simple and fast method (10 minutes) for simultaneous SAM and SAH quantification in plasma and blood samples is proposed based on the HPLC-PDA-MS technique. A new solid phase extraction procedure for blood samples is reported. The developed method proved to be highly sensitive (1.3 × 10 −9 molL −1 for SAM and 4.5 × 10 −10 molL −1 for SAH), repeatable (CV = 1.06% for SAM and CV = 0.92% for SAH), and reproducible (CV = 4.1% for SAM and CV = 3.77% for SAH).
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710903486385