Investigation of Induced Peak Phenomenon in Capillary Electrophoresis with Electrochemiluminescence Detection

Induced peak phenomenon in capillary zone electrophoresis with electrochemiluminescence detection for chiral separation of racemic phenylalanine mixture employing sulfated-β-cyclodextrin as chiral selector and acetonitrile as organic additive in the separation buffer was observed. Various experiment...

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Bibliographic Details
Published in:Analytical letters 2007-12, Vol.40 (18), p.3457-3471
Main Authors: Fang, Lanyun, Yin, Xue-Bo, Wang, Erkang
Format: Article
Language:English
Subjects:
acetonitrile
Analytical chemistry
Capillarity
capillary electrophoresis
Chemistry
Chromatographic methods and physical methods associated with chromatography
Complexation
Electrochemiluminescence
Electrophoresis
Exact sciences and technology
Induced peak
Mathematical analysis
Other chromatographic methods
Phenylalanine
Selectors
Separation
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Summary:Induced peak phenomenon in capillary zone electrophoresis with electrochemiluminescence detection for chiral separation of racemic phenylalanine mixture employing sulfated-β-cyclodextrin as chiral selector and acetonitrile as organic additive in the separation buffer was observed. Various experimental parameters influencing the intensity and the position of the induced peak were systematically investigated to find out the truth of the induced peak. Based on the experimental evidence, a reasonable mechanism involved in the formation of the induced peak was proposed. We found out the induced peak resulted from physical interactions between the components in the separation buffer and the injected sample during the electromigration process rather than chemical complexation interactions. Furthermore, suggestions to avoid the appearance of induced peak in capillary zone electrophoresis with electrochemiluminescence detection for chiral separations were presented.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710701689065