Semi-quantitative analysis of drugs of abuse, including tetrahydrocannabinol in hair using aqueous extraction and immunoassay

Abstract A semi-quantitative analytical screening procedure for the determination of cocaine, amphetamines, opiates, and delta-9-tetrahydrocannabinol in hair has been developed. The procedure employs an aqueous extraction buffer, uses only 10 mg of hair, requires 2 h of incubation for the extraction...

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Bibliographic Details
Published in:Forensic science international 2010-03, Vol.196 (1), p.70-73
Main Authors: Coulter, Cynthia, Tuyay, James, Taruc, Margaux, Moore, Christine
Format: Article
Language:English
Subjects:
Amphetamines - analysis
Biological and medical sciences
Buffers
Calibration
Cocaine - analysis
Dronabinol - analysis
Drug abuse
Drugs
Drugs of abuse
Enzyme-Linked Immunosorbent Assay
Enzymes
Extraction
Forensic medicine
Forensic science
Forensic sciences
Forensic Toxicology - methods
General aspects
Hair
Hair - chemistry
Hair analysis
Humans
Immunoassay
Investigative techniques, diagnostic techniques (general aspects)
Laboratories
Medical sciences
Medical screening
Methamphetamine
Narcotics - analysis
Pathology
Public health. Hygiene
Public health. Hygiene-occupational medicine
Screening
Substance Abuse Detection - methods
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Summary:Abstract A semi-quantitative analytical screening procedure for the determination of cocaine, amphetamines, opiates, and delta-9-tetrahydrocannabinol in hair has been developed. The procedure employs an aqueous extraction buffer, uses only 10 mg of hair, requires 2 h of incubation for the extraction to occur, and multiple drug classes can be screened using enzyme linked immunosorbent assays. Hair calibration standards were prepared around the recommended cut-off concentrations of the Society of Hair Testing. All drug classes showed excellent linearity over the concentration range tested, indicating that immunochemical screening can be used in a semi-quantitative mode for hair analysis using an aqueous buffer, rapid extraction and a small amount of hair.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2009.12.025