Determination of melamine and cyanuric acid in human urine by a liquid chromatography tandem mass spectrometry

Melamine was found to be the etiological factor for the urinary stones epidemic in infants and young children in China in 2008. Urine level of melamine and its analog cyanuric acid may be useful markers for the evaluation of toxic effects. Liquid chromatography tandem mass spectrometry methods for t...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-03, Vol.878 (9), p.758-762
Main Authors: Zhang, Mengqi, Li, Shuijun, Yu, Chengyin, Liu, Gangyi, Jia, Jingying, Lu, Chuan, He, Jian, Ma, Yinghua, Zhu, Jianmin, Yu, Chen
Format: Article
Language:English
Subjects:
Adult
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Child
Child, Preschool
Chromatography, High Pressure Liquid - methods
Cyanuric acid
Female
Fundamental and applied biological sciences. Psychology
General pharmacology
Human
Human urine
Humans
Infant
Infants
Linearity
Liquid chromatography
Liquid chromatography tandem mass spectrometry
Male
Medical sciences
Melamine
Middle Aged
Pharmacology. Drug treatments
Tandem Mass Spectrometry - methods
Triazines - urine
Urine
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Summary:Melamine was found to be the etiological factor for the urinary stones epidemic in infants and young children in China in 2008. Urine level of melamine and its analog cyanuric acid may be useful markers for the evaluation of toxic effects. Liquid chromatography tandem mass spectrometry methods for the individual determination of melamine and cyanuric acid in human urine are described. Using isotope labeled internal standards during liquid–liquid extraction, the method was fully validated by verifying specificity, linearity, LLOQ, intra- and inter-assay precision and accuracy, matrix effect, recovery and stability. Calibration curves with good linearity ( r = 0.9999) over the concentration range from 10 to 5000 ng/ml, intra-assay precision
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2010.01.020