Development of enzyme linked immunoassay for the simultaneous detection of carbaryl and metolcarb in different agricultural products

• Published in: Analytica chimica acta 2010-05, Vol.666 (1), p.76-82
• Main Authors: Sun, Jingwei, Dong, Tingting, Zhang, Yan, Wang, Shuo
• Format: Article
• Language: English
• Subjects: Agricultural products; Agriculture; Analytical chemistry; Animals; Antibodies - immunology; Assaying; Beverages - analysis; Carbaryl; Carbaryl - analysis; Carbaryl - immunology; Chemistry; Chromatographic methods and physical methods associated with chromatography; Correlation; Enzyme linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzymes; Enzymes - metabolism; Exact sciences and technology; Extraction; Feasibility Studies; Format; High performance liquid chromatography; Immunoassay; Malus - chemistry; Matrix effects; Metolcarb; Miscellaneous; Other chromatographic methods; Pesticide Residues - analysis; Pesticide Residues - immunology; Phenylcarbamates - analysis; Phenylcarbamates - immunology; Reproducibility of Results; Simultaneous determination; Time Factors; Tracers; Vegetables - chemistry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2010.03.051

A direct competitive enzyme linked immunosorbent assay in multi-enzyme tracers format for the simultaneous analysis of carbaryl and metolcarb in agricultural products is described in this study. The concentrations of coating antibodies and enzyme tracer were studied. Under the optimum conditions, the limits of detection of carbaryl and metolcarb were 0.15 μg L −1 and 1.2 μg L −1, respectively. Determination of carbaryl and metolcarb in fruit juices and vegetables was accomplished by simple, rapid and efficient extraction methods. Recoveries of spiked samples were great than 70%. Validation of the immunosorbent assay was conducted by comparison of results from high performance liquid chromatography (HPLC). The correlations between the data obtained using multi-enzyme tracers enzyme linked immunosorbent assay and high performance liquid chromatography were good. Results indicated that the new strategy for developing immunoassay for simultaneous quantitative determination of carbaryl and metolcarb residues was suitable in this study.