Selective inhibition of herpes simplex virus glycoprotein synthesis by a benz-amidinohydrazone derivative

1H-benz[f]indene-1.3(2H)dione-bis-amidinohydrazone (benzhydrazone) inhibited incorporation of 14C-glucosamine, 14C-fucose and 14C-mannose into glycoproteins of HEp-2 cells infected with various strains of herpes simplex virus 1 (HSV-1) and impaired RNA and protein synthesis to a low extent. These bi...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 1980-01, Vol.66 (3), p.179-191
Main Authors: Campadelli-Fiume, G, Sinibaldi-Vallebona, P, Cavrini, V, Mannini-Palenzona, A
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cricetinae
Cytopathogenic Effect, Viral - drug effects
Glycoproteins - biosynthesis
Hydrazones - pharmacology
Measles virus - growth & development
Paramyxoviridae - growth & development
RNA, Viral - biosynthesis
Simplexvirus - drug effects
Simplexvirus - growth & development
Simplexvirus - metabolism
Viral Proteins - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:1H-benz[f]indene-1.3(2H)dione-bis-amidinohydrazone (benzhydrazone) inhibited incorporation of 14C-glucosamine, 14C-fucose and 14C-mannose into glycoproteins of HEp-2 cells infected with various strains of herpes simplex virus 1 (HSV-1) and impaired RNA and protein synthesis to a low extent. These biochemical effects are very similar to those induced by glycosylation inhibitors such as tunicamycin, D-glucosamine and 2-deoxy-D-glucose. In contrast to these inhibitors, benzhydrazone reduced HSV glycoprotein synthesis selectively since it did not significantly modify i) the saccharide uptake into glycoproteins of uninfected and of Sindbis virus-infected cells, ii) viral growth and cell fusion in paramyxovirus-infected cells, two activities which depend on viral glycoprotein synthesis. Benzhydrazone had only minor effects on the overall metabolism of uninfected cells, since it did not alter cell growth rate, and amino acid, uridine, and hexose incorporations were about 80% those of untreated cells.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01314732