Rapid Screening of Lectins for Multivalency Effects with a Glycodendrimer Microarray

• Published in: Chembiochem : a European journal of chemical biology 2010-09, Vol.11 (13), p.1896-1904
• Main Authors: Parera Pera, Núria, Branderhorst, Hilbert M, Kooij, Raymond, Maierhofer, Caroline, van der Kaaden, Marjolein, Liskamp, Rob M.J, Wittmann, Valentin, Ruijtenbeek, Rob, Pieters, Roland J
• Format: Article
• Language: English
• Subjects: carbohydrates; Carbohydrates - chemistry; click chemistry; dendrimers; Dendrimers - chemical synthesis; Dendrimers - chemistry; inhibitors; Lectins - analysis; Microarray Analysis - methods; microarrays; Protein Binding; Spectrometry, Fluorescence
• ISSN: 1439-4227; 1439-7633
• DOI: 10.1002/cbic.201000340

Multivalency is an important phenomenon in protein-carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins.