Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks

Infection with duck circovirus (DuCV) is associated with growth retardation and developmental problems in farmed ducks. To detect DuCV-specific antibody in duck serum, an indirect enzyme-linked immunosorbent assay (iELISA) method was developed using the recombinant capsid protein antigen prepared by...

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Bibliographic Details
Published in:Veterinary microbiology 2010-09, Vol.145 (1), p.41-46
Main Authors: Liu, Shao-Ning, Zhang, Xing-Xiao, Zou, Jin-Feng, Xie, Zhi-Jing, Zhu, Yan-Li, Zhao, Qin, Zhou, En-min, Jiang, Shi-Jin
Format: Article
Language:English
Subjects:
Animals
antibodies
Biological and medical sciences
Blotting, Western - veterinary
Capsid protein (Cap)
Capsid Proteins - immunology
Circoviridae Infections - immunology
Circoviridae Infections - veterinary
Circoviridae Infections - virology
Circovirus
coat proteins
diagnostic techniques
disease detection
Duck circovirus
Duck circovirus (DuCV)
ducks
Ducks - virology
Electrophoresis, Polyacrylamide Gel - veterinary
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Fundamental and applied biological sciences. Psychology
Indirect ELISA
microbial genetics
Microbiology
Miscellaneous
pathogen identification
plasmid vectors
Poultry Diseases - diagnosis
Poultry Diseases - immunology
Poultry Diseases - virology
Prokaryotic expression
Recombinant Proteins - immunology
serodiagnosis
viral antigens
viral diseases of animals and humans
Viral Fusion Proteins - immunology
Virology
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Summary:Infection with duck circovirus (DuCV) is associated with growth retardation and developmental problems in farmed ducks. To detect DuCV-specific antibody in duck serum, an indirect enzyme-linked immunosorbent assay (iELISA) method was developed using the recombinant capsid protein antigen prepared by cloning the cap (Cap) gene of DuCV FJ0601 strain into pET-32a (+) vector and expressed in Escherichia coli. Using the optimized iELISA method, DuCV-specific antibodies were detected in 157 (12.96%) of 1211 samples obtained from 17 (89.47%) of 19 meat duck flocks aged from 25 to 40 days and in 89 (22.08%) of 403 samples obtained from 9 (75%) of 12 breeder flocks aged from 14 to 61 weeks. These results indicated that the iELISA method is useful for serological diagnosis of DuCV infection and epidemiological investigation.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2010.03.010