Directing the Morphology and Differentiation of Skeletal Muscle Cells Using Oriented Cellulose Nanowhiskers

Radially oriented submonolayer surfaces of 10−15 nm diameter cellulose nanowhiskers (CNWs) were prepared by spin-coating. The response of myoblasts (muscle cells) to the surfaces was assessed using atomic force microscopy (AFM), immunocytochemistry, and image analysis. Despite the small size of the...

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Bibliographic Details
Published in:Biomacromolecules 2010-09, Vol.11 (9), p.2498-2504
Main Authors: Dugan, James M, Gough, Julie E, Eichhorn, Stephen J
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
Cell Differentiation
Cells, Cultured
Cellulose - chemistry
Cellulose and derivatives
Exact sciences and technology
Focal Adhesions
Image Processing, Computer-Assisted
Immunoenzyme Techniques
Medical sciences
Mice
Microscopy, Atomic Force
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
Myoblasts - chemistry
Myoblasts - cytology
Myoblasts - metabolism
Nanotechnology
Natural polymers
Physicochemistry of polymers
Plasticizers
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Urochordata - cytology
Urochordata - metabolism
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Summary:Radially oriented submonolayer surfaces of 10−15 nm diameter cellulose nanowhiskers (CNWs) were prepared by spin-coating. The response of myoblasts (muscle cells) to the surfaces was assessed using atomic force microscopy (AFM), immunocytochemistry, and image analysis. Despite the small size of the CNWs, the myoblasts oriented along the CNW surfaces. Upon differentiation, the myoblasts produced striking radial patterns of myotubes, following the radial pattern of the CNWs. This facile method of nanopatterning surfaces may be applied where the directed growth of tissue is required and shows for the first time the potential of CNWs for tissue engineering applications.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm100684k