Development of nanoparticle probes for multiplex SERS imaging of cell surface proteins

Multiplexed SERS imaging of receptor proteins on the surface of mammalian cells has been carried out using functionalized silver nanoparticles. Deconvolution of four differently functionalized nanoparticles is readily achieved, and using this approach, receptor co-localization can be probed and prot...

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Bibliographic Details
Published in:Nanoscale 2010-08, Vol.2 (8), p.1413-1416
Main Authors: Kennedy, David C, Hoop, Kelly A, Tay, Li-Lin, Pezacki, John Paul
Format: Article
Language:English
Subjects:
Animals
Caveolin 3 - chemistry
Ligands
Membrane Proteins - chemistry
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Microscopy, Confocal
Myocytes, Cardiac - cytology
Rats
Receptors, Adrenergic, beta-2 - chemistry
Silver - chemistry
Spectrum Analysis, Raman
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Summary:Multiplexed SERS imaging of receptor proteins on the surface of mammalian cells has been carried out using functionalized silver nanoparticles. Deconvolution of four differently functionalized nanoparticles is readily achieved, and using this approach, receptor co-localization can be probed and protein-protein interactions can be elucidated at the surface of cells.
ISSN:2040-3364
2040-3372
DOI:10.1039/c0nr00122h