Interaction of PqqE and PqqD in the pyrroloquinoline quinone (PQQ) biosynthetic pathway links PqqD to the radical SAM superfamily

pqqD is one of six genes required for PQQ production in Klebsiella pneumoniae. Herein, we demonstrate that PqqD interacts specifically with the radical SAM enzyme PqqE, causing a perturbation in the electronic environment around the [4Fe-4S](+) clusters. This interaction redirects the role for PqqD...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2010-10, Vol.46 (37), p.7031-7033
Main Authors: Wecksler, Stephen R, Stoll, Stefan, Iavarone, Anthony T, Imsand, Erin M, Tran, Ha, Britt, R David, Klinman, Judith P
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Free Radicals - chemistry
Free Radicals - metabolism
Klebsiella pneumoniae - enzymology
PQQ Cofactor - biosynthesis
PQQ Cofactor - chemistry
S-Adenosylmethionine - chemistry
S-Adenosylmethionine - metabolism
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Summary:pqqD is one of six genes required for PQQ production in Klebsiella pneumoniae. Herein, we demonstrate that PqqD interacts specifically with the radical SAM enzyme PqqE, causing a perturbation in the electronic environment around the [4Fe-4S](+) clusters. This interaction redirects the role for PqqD in PQQ biosynthesis.
ISSN:1359-7345
1364-548X
DOI:10.1039/c0cc00968g