Inactivation of An Agent of Human Non-A, Non-B Hepatitis by Formalin

Samples of serum (0.1 ml each) containing an agent of human non-A, non-B hepatitis of documented infectivity were incubated with formalin in a concentration of 1:1,000 at 37 C for 96 hr. Three colony-born infant chimpanzees were then inoculated with this formalin-treated serum; one received a single...

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Bibliographic Details
Published in:The Journal of infectious diseases 1980-11, Vol.142 (5), p.767-770
Main Authors: Tabor, Edward, Gerety, Robert J.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Biopsies
Chimpanzees
Disease transmission
Formaldehyde - pharmacology
Hepatitis
Hepatitis C - pathology
Hepatitis C - prevention & control
Hepatitis Viruses - drug effects
Hepatitis, Viral, Human - prevention & control
Histology
Inoculation
Inoculum
Liver
Liver - pathology
Medical Microbiology
Pan troglodytes
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Summary:Samples of serum (0.1 ml each) containing an agent of human non-A, non-B hepatitis of documented infectivity were incubated with formalin in a concentration of 1:1,000 at 37 C for 96 hr. Three colony-born infant chimpanzees were then inoculated with this formalin-treated serum; one received a single intravenous inoculation, and two received two subcutaneous inoculations one month apart. A fourth uninoculated chimpanzee served as a control. None developed recognizable non-A, non-B hepatitis during seven months of observation, as judged by normal aminotransferase levels in weekly serum samples, normal liver histology in liver biopsy specimens, and the absence of non-A, non-B hepatitis-associated antigen and antibody in their sera. All four chimpanzees were subsequently shown to be susceptible to non-A, non-B hepatitis when challenged with 0.1 ml of the untreated infectious serum 31 weeks after the initial inoculations.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/142.5.767