Long-term Stability of Circulating Nucleosomes in Serum

Circulating nucleosomes, complexes of DNA and histones, are recognized as a potential new diagnostic tool for therapy monitoring of cancer disease and as prognostic marker in other acute diseases. Because many studies are carried out retrospectively on serum samples collected earlier, we tested the...

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Bibliographic Details
Published in:Anticancer research 2010-05, Vol.30 (5), p.1613-1615
Main Authors: HOLDENRIEDER, Stefan, VON PAWEL, Joachim, NAGEL, Dorothea, STIEBER, Petra
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood Preservation - methods
Cell-Free System
DNA - chemistry
DNA - metabolism
Enzyme-Linked Immunosorbent Assay - methods
Freezing
Histones - metabolism
Humans
Kinetics
Medical sciences
Neoplasms - blood
Neoplasms - metabolism
Nucleosomes - metabolism
Specimen Handling - methods
Time Factors
Tumors
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Summary:Circulating nucleosomes, complexes of DNA and histones, are recognized as a potential new diagnostic tool for therapy monitoring of cancer disease and as prognostic marker in other acute diseases. Because many studies are carried out retrospectively on serum samples collected earlier, we tested the stability of nucleosomes during long-term storage at -70 degrees C. Never-thawed aliquots of 154 stabilized serum samples from cancer patients which had been stored between 11/1999 and 07/2001 at -70 degrees C were analyzed by ELISA six to nine months after collection and for a second time in 11/2006, with a median interval of 64.8 months (+/-5.5 months) between the measurements. Nucleosome levels of the second measurement (median 220.0 ng/ml) were significantly lower than those of the first measurement (354.0 ng/ml; p
ISSN:0250-7005
1791-7530