Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout

The current research aims to determine alterations in gene expression and enzymatic activity of fish antioxidant metabolism in response to pesticide administration. To this end, three different deltamethrin concentrations (0.25, 1, 2.5μg/L) were administrated to rainbow trout (Oncorhynchus mykiss) a...

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Published in:Comparative biochemistry and physiology. Toxicology & pharmacology 2010-08, Vol.152 (2), p.215-223
Main Authors: Ceyhun, Saltuk Buğrahan, Şentürk, Murat, Ekinci, Deniz, Erdoğan, Orhan, Çiltaş, Abdulkadir, Kocaman, Esat Mahmut
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Deltamethrin
Gene Expression - drug effects
Gills - enzymology
Glutathione
Glutathione Reductase - metabolism
Hsp70
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Insecticides - toxicity
Kidney - enzymology
Liver - enzymology
Muscles - enzymology
Nitriles - toxicity
Oncorhynchus mykiss
Oncorhynchus mykiss - genetics
Oncorhynchus mykiss - metabolism
Oxidative Stress
Pentose phosphate
Pyrethrins - toxicity
Stress
Water Pollutants, Chemical - toxicity
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Summary:The current research aims to determine alterations in gene expression and enzymatic activity of fish antioxidant metabolism in response to pesticide administration. To this end, three different deltamethrin concentrations (0.25, 1, 2.5μg/L) were administrated to rainbow trout (Oncorhynchus mykiss) at different time intervals (6, 12, 24, 48 and 72h) in order to observe the influences of the pesticide on the activity of glutathione reductase, glucose 6-phosphate dehydrogenase, 6-ghosphogluconate dehydrogenase, and the expression of Hsp70 gene. We observed that the activities of the enzymes decreased with increasing deltamethrin concentrations and exposure time. The pesticide had more inhibitory effects on gill enzymes than those of muscle, liver and kidney. In addition, we detected that deltamethrin increased the expression of the stress-related protein Hsp70 with significant fold-chance values. The efficiency rate was 96.4% which is equal to 1.96 calculated via conversion formula used to calculate fold-chance value. We conclude that deltamethrin causes oxidative stress in fish both at protein and mRNA levels.
ISSN:1532-0456
1878-1659
DOI:10.1016/j.cbpc.2010.04.008