Pollen-pistil compatibility relationships in some Iranian almond (Prunus dulcis, Batch) genotypes as revealed by PCR analysis

The identification of pollen-pistil compatibility relationships among almond cultivars and genotypes is very important for breeders and growers. In the present study, PCR based technique was used to identify S-alleles in 10 late blooming almond genotypes. In total, 19 alleles were amplified by five...

Full description

Saved in:
Bibliographic Details
Published in:African journal of biotechnology 2010-06, Vol.9 (23), p.3338-3341
Main Authors: Sharafi, Y, Hajilou, J, Mohammadi, SAG, Dadpour, M R, Eskandari, S
Format: Article
Language:English
Subjects:
Prunus dulcis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The identification of pollen-pistil compatibility relationships among almond cultivars and genotypes is very important for breeders and growers. In the present study, PCR based technique was used to identify S-alleles in 10 late blooming almond genotypes. In total, 19 alleles were amplified by five primer pairs in the studied genotypes. The size of bands ranged between 480 - 2000 bp. Seven S-alleles were amplified using AS1II/AMYC5R primer pair, whereas each of the Alsc11/AMYC5R, Pru-C2/Pru-C4R, Pru-C2/Pru-C5R and Pru-C2/Pru-C6R primer pairs amplified nine different S-alleles. Based on S-allele patterns, all of the studied genotypes were identified as self-incompatible. However, some of the genotypes had only one similar S-allele, all of the genotypes could be used in establishment of commercial orchards based on their blooming times.
ISSN:1684-5315
1684-5315